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  • Wiley  (5)
  • Then, Johannes  (5)
  • 1
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    Online Resource
    A disulfide bridge in the calcium binding site of a polyester hydrolase increases its thermal stability and activity against polyethylene terephthalate
    Wiley ; 2016
    In:  FEBS Open Bio Vol. 6, No. 5 ( 2016-05), p. 425-432
    Details
    In: FEBS Open Bio, Wiley, Vol. 6, No. 5 ( 2016-05), p. 425-432
    Abstract: Elevated reaction temperatures are crucial for the efficient enzymatic degradation of polyethylene terephthalate (PET). A disulfide bridge was introduced to the polyester hydrolase TfCut2 to substitute its calcium binding site. The melting point of the resulting variant increased to 94.7 °C (wild‐type TfCut2: 69.8 °C) and its half‐inactivation temperature to 84.6 °C (TfCut2: 67.3 °C). The variant D204C‐E253C‐D174R obtained by introducing further mutations at vicinal residues showed a temperature optimum between 75 and 80 °C compared to 65 and 70 °C of the wild‐type enzyme. The variant caused a weight loss of PET films of 25.0 ± 0.8% (TfCut2: 0.3 ± 0.1%) at 70 °C after a reaction time of 48 h. The results demonstrate that a highly efficient and calcium‐independent thermostable polyester hydrolase can be obtained by replacing its calcium binding site with a disulfide bridge.
    Type of Medium: Online Resource
    ISSN: 2211-5463 , 2211-5463
    URL: Article
    DOI: 10.1002/2211-5463.12053
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2651702-4
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  • 2
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    Online Resource
    Engineered bacterial polyester hydrolases efficiently degrade polyethylene terephthalate due to relieved product inhibition
    Wiley ; 2016
    In:  Biotechnology and Bioengineering Vol. 113, No. 8 ( 2016-08), p. 1658-1665
    Details
    In: Biotechnology and Bioengineering, Wiley, Vol. 113, No. 8 ( 2016-08), p. 1658-1665
    Abstract: Recent studies on the enzymatic degradation of synthetic polyesters have shown the potential of polyester hydrolases from thermophilic actinomycetes for modifying or degrading polyethylene terephthalate (PET). TfCut2 from Thermobifida fusca KW3 and LC‐cutinase (LCC) isolated from a compost metagenome are remarkably active polyester hydrolases with high sequence and structural similarity. Both enzymes exhibit an exposed active site in a substrate binding groove located at the protein surface. By exchanging selected amino acid residues of TfCut2 involved in substrate binding with those present in LCC, enzyme variants with increased PET hydrolytic activity at 65°C were obtained. The highest activity in hydrolyzing PET films and fibers were detected with the single variant G62A and the double variant G62A/I213S. Both variants caused a weight loss of PET films of more than 42% after 50 h of hydrolysis, corresponding to a 2.7‐fold increase compared to the wild type enzyme. Kinetic analysis based on the released PET hydrolysis products confirmed the superior hydrolytic activity of G62A with a fourfold higher hydrolysis rate constant and a 1.5‐fold lower substrate binding constant than those of the wild type enzyme. Mono‐(2‐hydroxyethyl) terephthalate is a strong inhibitor of TfCut2. A determination of the Rosetta binding energy suggested a reduced interaction of G62A with 2PET, a dimer of the PET monomer ethylene terephthalate. Indeed, G62A revealed a 5.5‐fold lower binding constant to the inhibitor than the wild type enzyme indicating that its increased PET hydrolysis activity is the result of a relieved product inhibition by mono‐(2‐hydroxyethyl) terephthalate. Biotechnol. Bioeng. 2016;113: 1658–1665. © 2016 Wiley Periodicals, Inc.
    Type of Medium: Online Resource
    ISSN: 0006-3592 , 1097-0290
    URL: Issue
    URL: Article
    DOI: 10.1002/bit.v113.8
    DOI: 10.1002/bit.25941
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 1480809-2
    detail.hit.zdb_id: 280318-5
    SSG: 12
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  • 3
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    Online Resource
    A dual enzyme system composed of a polyester hydrolase and a carboxylesterase enhances the biocatalytic degradation of polyethylene terephthalate films
    Wiley ; 2016
    In:  Biotechnology Journal Vol. 11, No. 8 ( 2016-08), p. 1082-1087
    Details
    In: Biotechnology Journal, Wiley, Vol. 11, No. 8 ( 2016-08), p. 1082-1087
    Abstract: TfCut2 from Thermobifida fusca KW3 and the metagenome‐derived LC‐cutinase are bacterial polyester hydrolases capable of efficiently degrading polyethylene terephthalate (PET) films. Since the enzymatic PET hydrolysis is inhibited by the degradation intermediate mono‐(2‐hydroxyethyl) terephthalate (MHET), a dual enzyme system consisting of a polyester hydrolase and the immobilized carboxylesterase TfCa from Thermobifida fusca KW3 was employed for the hydrolysis of PET films at 60°C. HPLC analysis of the reaction products obtained after 24 h of hydrolysis showed an increased amount of soluble products with a lower proportion of MHET in the presence of the immobilized TfCa. The results indicated a continuous hydrolysis of the inhibitory MHET by the immobilized TfCa and demonstrated its advantage as a second biocatalyst in combination with a polyester hydrolase for an efficient degradation oft PET films. The dual enzyme system with LC‐cutinase produced a 2.4‐fold higher amount of degradation products compared to TfCut2 after a reaction time of 24 h confirming the superior activity of his polyester hydrolase against PET films.
    Type of Medium: Online Resource
    ISSN: 1860-6768 , 1860-7314
    URL: Issue
    URL: Article
    DOI: 10.1002/biot.v11.8
    DOI: 10.1002/biot.201600008
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2214038-4
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  • 4
    Online Resource
    Online Resource
    Ca 2+ and Mg 2+ binding site engineering increases the degradation of polyethylene terephthalate films by polyester hydrolases from Thermobifida fusca
    Wiley ; 2015
    In:  Biotechnology Journal Vol. 10, No. 4 ( 2015-04), p. 592-598
    Details
    In: Biotechnology Journal, Wiley, Vol. 10, No. 4 ( 2015-04), p. 592-598
    Abstract: Several bacterial polyester hydrolases are able to hydrolyze the synthetic polyester polyethylene terephthalate (PET). For an efficient enzymatic degradation of PET, reaction temperatures close to the glass transition temperature of the polymer need to be applied. The esterases TfH, BTA2, Tfu_0882, TfCut1, and TfCut2 produced by the thermophilic actinomycete Thermobifida fusca exhibit PET‐hydrolyzing activity. However, these enzymes are not sufficiently stable in this temperature range for an efficient degradation of post‐consumer PET materials. The addition of Ca 2+ or Mg 2+ cations to the enzymes resulted in an increase of their melting points between 10.8 and 14.1°C determined by circular dichroism spectroscopy. The thermostability of the polyester hydrolases was sufficient to degrade semi‐crystalline PET films at 65°C in the presence of 10 mM Ca 2+ and 10 mM Mg 2+ resulting in weight losses of up to 12.9% after a reaction time of 48 h. The residues Asp174, Asp204, and Glu253 were identified by molecular dynamics simulations as potential binding residues for the two cations in TfCut2. This was confirmed by their substitution with arginine, resulting in a higher thermal stability of the corresponding enzyme variants. The generated variants of TfCut2 represent stabilized catalysts suitable for PET hydrolysis reactions performed in the absence of Ca 2+ or Mg 2+ .
    Type of Medium: Online Resource
    ISSN: 1860-6768 , 1860-7314
    URL: Issue
    URL: Article
    DOI: 10.1002/biot.v10.4
    DOI: 10.1002/biot.201400620
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 2214038-4
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  • 5
    Online Resource
    Online Resource
    Effect of Tris, MOPS, and phosphate buffers on the hydrolysis of polyethylene terephthalate films by polyester hydrolases
    Wiley ; 2016
    In:  FEBS Open Bio Vol. 6, No. 9 ( 2016-09), p. 919-927
    Details
    In: FEBS Open Bio, Wiley, Vol. 6, No. 9 ( 2016-09), p. 919-927
    Abstract: The enzymatic degradation of polyethylene terephthalate (PET) occurs at mild reaction conditions and may find applications in environmentally friendly plastic waste recycling processes. The hydrolytic activity of the homologous polyester hydrolases LC cutinase (LCC) from a compost metagenome and TfCut2 from Thermobifida fusca KW3 against PET films was strongly influenced by the reaction medium buffers tris(hydroxymethyl)aminomethane (Tris), 3‐( N ‐morpholino)propanesulfonic acid (MOPS), and sodium phosphate. LCC showed the highest initial hydrolysis rate of PET films in 0.2 m Tris, while the rate of TfCut2 was 2.1‐fold lower at this buffer concentration. At a Tris concentration of 1 m , the hydrolysis rate of LCC decreased by more than 90% and of TfCut2 by about 80%. In 0.2 m MOPS or sodium phosphate buffer, no significant differences in the maximum initial hydrolysis rates of PET films by both enzymes were detected. When the concentration of MOPS was increased to 1 m , the hydrolysis rate of LCC decreased by about 90%. The activity of TfCut2 remained low compared to the increasing hydrolysis rates observed at higher concentrations of sodium phosphate buffer. In contrast, the activity of LCC did not change at different concentrations of this buffer. An inhibition study suggested a competitive inhibition of TfCut2 and LCC by Tris and MOPS. Molecular docking showed that Tris and MOPS interfered with the binding of the polymeric substrate in a groove located at the protein surface. A comparison of the K i values and the average binding energies indicated MOPS as the stronger inhibitor of the both enzymes.
    Type of Medium: Online Resource
    ISSN: 2211-5463 , 2211-5463
    URL: Article
    DOI: 10.1002/2211-5463.12097
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2651702-4
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