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  • Wiley  (6)
  • Teng, Sing Tung  (6)
  • 1
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    Three novel species in the P seudo‐nitzschia pseudodelicatissima complex: P .  batesiana sp. nov., P . lundholmiae sp. nov., and P . fukuyoi sp. nov. ( B acillariophyceae) from the S trait of M alacca, M alaysia
    Wiley ; 2013
    In:  Journal of Phycology Vol. 49, No. 5 ( 2013-10), p. 902-916
    Details
    In: Journal of Phycology, Wiley, Vol. 49, No. 5 ( 2013-10), p. 902-916
    Abstract: A study on the morphology and phylogeny of 18 strains of P seudo‐nitzschia established from the S trait of M alacca, P eninsular M alaysia, was undertaken. Morphological data combined with molecular evidence show that they constitute three new species, for which the names, P . batesiana sp. nov., P . lundholmiae sp. nov., and P . fukuyoi sp. nov., are proposed. The three new species closely resemble species in the P . pseudodelicatissima complex sensu lato . Morphologically, P . batesiana differs from other species in the complex by having a smaller part of cell overlapping in the chain, whereas P . lundholmiae differs by having fewer poroid sectors and P . fukuyoi by having a distinct type of poroid sectors. Nucleotide sequences of the LSU rDNA (D1–D3) of the three new species reveal significant nucleotide sequence divergence (0.1%–9.3%) from each other and from other species in the P . pseudodelicatissima complex s.l . The three species are phylogenetically closely related to species in the P . pseudodelicatissima complex, with P . batesiana appearing as a sister taxon to P . circumpora , P . caciantha , and P . subpacifica ; whereas P . lundholmiae and P . fukuyoi are more closely related to P . pseudodelicatissima and P . cuspidata . The three species show 2–3 compensatory base changes ( CBCs ) in their ITS 2 transcripts when compared to the closely related species. The ITS 2 with its structural information has proven its robustness in constructing a better resolved phylogenetic framework for P seudo‐nitzschia .
    Type of Medium: Online Resource
    ISSN: 0022-3646 , 1529-8817
    URL: Issue
    URL: Article
    DOI: 10.1111/jpy.2013.49.issue-5
    DOI: 10.1111/jpy.12101
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 281226-5
    detail.hit.zdb_id: 1478748-9
    SSG: 12
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  • 2
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    Phylogeny and species delineation in the marine diatom Pseudo‐nitzschia (Bacillariophyta) using cox1 , LSU , and ITS 2 rRNA genes: A perspective in character evolution
    Wiley ; 2018
    In:  Journal of Phycology Vol. 54, No. 2 ( 2018-04), p. 234-248
    Details
    In: Journal of Phycology, Wiley, Vol. 54, No. 2 ( 2018-04), p. 234-248
    Abstract: Analyses of the mitochondrial cox1 , the nuclear‐encoded large subunit ( LSU ), and the internal transcribed spacer 2 ( ITS 2) RNA coding region of Pseudo‐nitzschia revealed that the P. pseudodelicatissima complex can be phylogenetically grouped into three distinct clades (Groups I–III), while the P. delicatissima complex forms another distinct clade (Group IV) in both the LSU and ITS 2 phylogenetic trees. It was elucidated that comprehensive taxon sampling (sampling of sequences), selection of appropriate target genes and outgroup, and alignment strategies influenced the phylogenetic accuracy. Based on the genetic divergence, ITS 2 resulted in the most resolved trees, followed by cox1 and LSU . The morphological characters available for Pseudo‐nitzschia , although limited in number, were overall in agreement with the phylogenies when mapped onto the ITS 2 tree. Information on the presence/absence of a central nodule, number of rows of poroids in each stria, and of sectors dividing the poroids mapped onto the ITS 2 tree revealed the evolution of the recently diverged species. The morphologically based species complexes showed evolutionary relevance in agreement with molecular phylogeny inferred from ITS 2 sequence–structure data. The data set of the hypervariable region of ITS 2 improved the phylogenetic inference compared to the cox1 and LSU data sets. The taxonomic status of P. cuspidata and P. pseudodelicatissima requires further elucidation.
    Type of Medium: Online Resource
    ISSN: 0022-3646 , 1529-8817
    URL: Issue
    URL: Article
    DOI: 10.1111/jpy.2018.54.issue-2
    DOI: 10.1111/jpy.12620
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 281226-5
    detail.hit.zdb_id: 1478748-9
    SSG: 12
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  • 3
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    A non‐toxigenic but morphologically and phylogenetically distinct new species of Pseudo‐nitzschia , P. sabit sp. nov. (Bacillariophyceae)
    Wiley ; 2015
    In:  Journal of Phycology Vol. 51, No. 4 ( 2015-08), p. 706-725
    Details
    In: Journal of Phycology, Wiley, Vol. 51, No. 4 ( 2015-08), p. 706-725
    Abstract: A new species of Pseudo‐nitzschia (Bacillariophyceae) is described from plankton samples collected from Port Dickson (Malacca Strait, Malaysia) and Manzanillo Bay (Colima, Mexico). The species possesses a distinctive falcate cell valve, from which they form sickle‐like colonies in both environmental samples and cultured strains. Detailed observation of frustules under TEM revealed ultrastructure that closely resembles P. decipiens , yet the new species differs by the valve shape and greater ranges of striae and poroid densities. The species is readily distinguished from the curve‐shaped P. subcurvata by the presence of a central interspace. The morphological distinction is further supported by phylogenetic discrimination. We sequenced and analyzed the nuclear ribosomal RNA genes in the LSU and the second internal transcribed spacer, including its secondary structure, to infer the phylogenetic relationship of the new species with its closest relatives. The results revealed a distinct lineage of the new species, forming a sister cluster with its related species, P. decipiens and P. galaxiae , but not with P. subcurvata . We examined the domoic acid ( DA ) production of five cultured strains from Malaysia by Liquid chromatography‐mass spectrometry (LC‐MS), but they showed no detectable DA . Here, we present the taxonomic description of the vegetative cells, document the sexual reproduction, and detail the molecular phylogenetics of Pseudo‐nitzschia sabit sp. nov.
    Type of Medium: Online Resource
    ISSN: 0022-3646 , 1529-8817
    URL: Issue
    URL: Article
    DOI: 10.1111/jpy.2015.51.issue-4
    DOI: 10.1111/jpy.12313
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 281226-5
    detail.hit.zdb_id: 1478748-9
    SSG: 12
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  • 4
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    Online Resource
    Quantitative real‐time PCR detection of a harmful unarmoured dinoflagellate, Karlodinium australe ( D inophyceae)
    Wiley ; 2017
    In:  Phycological Research Vol. 65, No. 4 ( 2017-10), p. 291-298
    Details
    In: Phycological Research, Wiley, Vol. 65, No. 4 ( 2017-10), p. 291-298
    Abstract: We investigated a harmful algal bloom ( HAB ) associated with the massive fish kills in J ohor S trait, M alaysia, which recurred a year after the first incident in 2014. This incident has urged for the need to have a rapid and precise method in HAB monitoring. In this study, we develop a SYBR green‐based real‐time PCR (q PCR ) to detect the culpable dinoflagellate species, Karlodinium australe . Species‐specific q PCR primers were designed in the gene region of the second internal transcribed spacer of the ribosomal RNA gene (r DNA ). The species specificity of the primers designed was evaluated by screening on the non‐target species ( Karlodinium veneficum , Takayama spp., and Karenia spp.) and no cross‐detection was observed. The extractable gene copies per cell of K . australe determined in this study were 19 998 ± 505 ( P 〈 0.0001). Estimation of cell densities by qPCR in the experimental spiked samples showed high correlation with data determined microscopically ( R 2 = 0.93). Using the qPCR assay developed in this study, we successfully detected the 2015 bloom species as K. australe . Single‐cell PCR and r DNA sequencing from the field samples further confirmed the finding. With the sensitivity as low as five cells, the q PCR assay developed in this study could effectively and rapidly detect cells of K. australe in the environmental samples for monitoring purpose.
    Type of Medium: Online Resource
    ISSN: 1322-0829 , 1440-1835
    URL: Issue
    URL: Article
    DOI: 10.1111/pre.2017.65.issue-4
    DOI: 10.1111/pre.12186
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 2020835-2
    SSG: 12
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  • 5
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    Online Resource
    Pseudo‐nitzschia nanaoensis sp. nov. (Bacillariophyceae) from the Chinese coast of the South China Sea
    Wiley ; 2018
    In:  Journal of Phycology Vol. 54, No. 6 ( 2018-12), p. 918-922
    Details
    In: Journal of Phycology, Wiley, Vol. 54, No. 6 ( 2018-12), p. 918-922
    Abstract: Pseudo‐nitzschia nanaoensis sp. nov. is described from waters around Nan'ao Island (South China Sea), using morphological data and molecular evidence. This species is morphologically most similar to P. brasiliana , but differs by a denser arrangement of fibulae, interstriae, and poroids, as well as by the structure of the valvocopula and the narrow second band. Pseudo‐nitzschia nanaoensis constitutes a monophyletic lineage and is well differentiated from other species on the LSU and ITS 2 sequence‐structure trees. Pseudo‐nitzschia nanaoensis makes up the basal node on the LSU tree, and forms a sister clade with a group of P. pungens and P. multiseries on the ITS2 tree. The ability of cultured strains to produce domoic acid was assessed, including its possible induction by the presence of a copepod and brine shrimp, by liquid chromatography–tandem mass spectrometry. However, no strains showed detectable domoic acid.
    Type of Medium: Online Resource
    ISSN: 0022-3646 , 1529-8817
    URL: Issue
    URL: Article
    DOI: 10.1111/jpy.2018.54.issue-6
    DOI: 10.1111/jpy.12791
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 281226-5
    detail.hit.zdb_id: 1478748-9
    SSG: 12
    Location Call Number Limitation Availability
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  • 6
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    Online Resource
    Morphology, ultrastructure, and molecular phylogeny of Wangodinium sinense gen. et sp. nov. (Gymnodiniales, Dinophyceae) and revisiting of Gymnodinium dorsalisulcum and Gymnodinium impudicum
    Wiley ; 2018
    In:  Journal of Phycology Vol. 54, No. 5 ( 2018-10), p. 744-761
    Details
    In: Journal of Phycology, Wiley, Vol. 54, No. 5 ( 2018-10), p. 744-761
    Abstract: The genus Gymnodinium includes many morphologically similar species, but molecular phylogenies show that it is polyphyletic. Eight strains of Gymnodinium impudicum , Gymnodinium dorsalisulcum and a novel Gymnodinium ‐like species from Chinese and Malaysian waters and the Mediterranean Sea were established. All of these strains were examined with light microscopy, scanning electron microscopy and transmission electron microscopy. SSU , LSU and internal transcribed spacers rDNA sequences were obtained. A new genus, Wangodinium , was erected to incorporate strains with a loop‐shaped apical structure complex ( ASC ) comprising two rows of amphiesmal vesicles, here referred to as a new type of ASC . The chloroplasts of Wangodinium sinense are enveloped by two membranes. Pigment analysis shows that peridinin is the main accessory pigment in W. sinense . Wangodinium differs from other genera mainly in its unique ASC , and additionally differs from Gymnodinium in the absence of nuclear chambers, and from Lepidodinium in the absence of Chl b and nuclear chambers. New morphological information was provided for G. dorsalisulcum and G. impudicum , e.g., a short sulcal intrusion in G. dorsalisulcum ; nuclear chambers in G. impudicum and G. dorsalisulcum ; and a chloroplast enveloped by two membranes in G. impudicum . Molecular phylogeny was inferred using maximum likelihood and Bayesian inference with independent SSU and LSU rDNA sequences. Our results support the classification of Wangodinium within the Gymnodiniales sensu stricto clade and it is close to Lepidodinium . Our results also support the close relationship among G. dorsalisulcum , G. impudicum, and Barrufeta . Further research is needed to assign these Gymnodinium species to Barrufeta or to erect new genera.
    Type of Medium: Online Resource
    ISSN: 0022-3646 , 1529-8817
    URL: Issue
    URL: Article
    DOI: 10.1111/jpy.2018.54.issue-5
    DOI: 10.1111/jpy.12780
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 281226-5
    detail.hit.zdb_id: 1478748-9
    SSG: 12
    Location Call Number Limitation Availability
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