In:
American Journal of Physiology-Cell Physiology, American Physiological Society, Vol. 295, No. 5 ( 2008-11), p. C1427-C1433
Abstract:
Accumulating evidence has indicated that mast cells can modulate a wide variety of immune responses. Migration and adhesion play a critical role in regulation of tissue mast cell function, in particular, under inflammatory conditions. We previously demonstrated that prostaglandin (PG) E 2 stimulates adhesion of a mouse mastocytoma cell line, P-815, to the Arg-Gly-Asp (RGD)-enriched matrix through cooperation between two PGE 2 receptor subtypes: EP3 and EP4 (Hatae N, Kita A, Tanaka S, Sugimoto Y, Ichikawa A. J Biol Chem 278: 17977–17981, 2003). We here investigated PGE 2 -induced adhesion of IL-3-dependent bone marrow-derived cultured mast cells (BMMCs). In contrast to the elevated cAMP-dependent adhesion of P-815 cells, EP3-mediated Ca 2+ mobilization plays a pivotal role in PGE 2 -induced adhesion of BMMCs. Adhesion and Ca 2+ mobilization induced by PGE 2 were abolished in the Ptger3 −/− BMMCs and were significantly suppressed by treatment with pertussis toxin, a phospholipase C inhibitor, U-73122, and a store-operated Ca 2+ channel inhibitor, SKF 36965, indicating the involvement of G i -mediated Ca 2+ influx. We then investigated PGE 2 -induced adhesion of peritoneal mast cells to the RGD-enriched matrix. EP3 subtype was found to be the dominant PGE receptor that expresses in mouse peritoneal mast cells. PGE 2 induced adhesion of the peritoneal mast cells of the Ptger3 +/+ mice, but not that of the Ptger3 −/− mice. In rat peritoneal mast cells, PGE 2 or an EP3 agonist stimulated both Ca 2+ mobilization and adhesion to the RGD-enriched matrix. These results suggested that the EP3 subtype plays a pivotal role in PGE 2 -induced adhesion of murine mast cells to the RGD-enriched matrix through Ca 2+ mobilization.
Type of Medium:
Online Resource
ISSN:
0363-6143
,
1522-1563
DOI:
10.1152/ajpcell.00218.2008
Language:
English
Publisher:
American Physiological Society
Publication Date:
2008
detail.hit.zdb_id:
1477334-X
SSG:
12
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