In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 60, No. 12 ( 2010-12-01), p. 2803-2807
Abstract:
Gram-reaction-positive, aerobic, non-spore-forming, irregular rod-shaped bacteria, designated AHU1821 T and AHU1820, were isolated from an ice wedge in the Fox permafrost tunnel, Alaska. The strains were psychrophilic, growing at −5 to 27 °C. Phylogenetic analysis of the 16S rRNA and gyrB gene sequences indicated that the ice-wedge isolates formed a clade distinct from other mycolic-acid-containing bacteria within the suborder Corynebacterineae . The cell wall of strains AHU1821 T and AHU1820 contained meso -diaminopimelic acid, arabinose and galactose, indicating chemotype IV. The muramic acids in the peptidoglycan were glycolated. The predominant menaquinone was MK-9(H 2 ). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and an unidentified glycolipid. The major fatty acids were hexadecenoic acid (C 16 : 1 ), hexadecanoic acid (C 16 : 0 ), octadecenoic acid (C 18 : 1 ) and tetradecanoic acid (C 14 : 0 ). Tuberculostearic acid was present in relatively small amounts (1 %). Strains AHU1821 T and AHU1820 contained mycolic acids with 42–52 carbons. The DNA G+C content of the two strains was 69.3–71.6 mol% ( T m ). 16S rRNA, rpoB and recA gene sequences were identical between strains AHU1821 T and AHU1820 and those of the gyrB gene showed 99.9 % similarity. Based on phylogenetic and phenotypic evidence, strains AHU1821 T and AHU1820 represent a single novel species of a novel genus, for which the name Tomitella biformata gen. nov., sp. nov. is proposed. The type strain of Tomitella biformata is AHU1821 T (=DSM 45403 T =NBRC 106253 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.017962-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2010
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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