In:
FEBS Letters, Wiley, Vol. 342, No. 1 ( 1994-03-28), p. 29-32
Abstract:
Complement receptor type 3 (CR3)‐mediated cellular responses in guinea pig macrophages were investigated by using zymosan and serum‐opsonized zymosan (SOZ) as the multivalent ligand for CR3. The ingestion of zymosan and SOZ was accompanied by O 2 − generation and arachidonate release. These responses were suppressed by prior exposure of macrophages to pertussis toxin (PT). Opsonization of zymosan gave rise to more than 6‐fold activation of the ingestion, whereas the magnitude of either arachinonate release or O 2 − generation was unchanged. The Fab' fragment of anti‐Z‐1, a monoclonal antibody specific for the α chain of guinea pig CR3, inhibited the ingestion of zymosan by 60% without affecting zymosan‐induced arachidonate release and O 2 − generation. These data suggested that there might be at least two functionally distinct binding sites for zymosan. O 2 − generation and arachidonate release might be regulated through one site and phagocytosis another. Both sites should be coupled to PT‐sensitive GTP binding protein.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(94)80578-4
Language:
English
Publisher:
Wiley
Publication Date:
1994
detail.hit.zdb_id:
1460391-3
SSG:
12
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