In:
American Journal of Reproductive Immunology, Wiley, Vol. 68, No. 6 ( 2012-12), p. 491-498
Abstract:
The aim of this study is to evaluate the expression and regulation of proprotein convertase subtilisin/kexin ( PCSK ) 6, which is known to be an important factor in the production of bone morphogenetic protein ( BMP ) cytokines in human ovary. Method of study The localization of PCSK 6 protein in normal human ovaries was examined by immunohistochemistry. Human granulosa cells (GC), obtained from 34 patients undergoing ovarian stimulation for in vitro fertilization, were cultured with BMP‐2, BMP‐6, BMP‐7, BMP‐15, growth differentiation factor (GDF)‐9, and activin‐A with or without FSH. PCSK 6 mRNA expression level was evaluated by quantitative real‐time reverse transcription and polymerase chain reaction (RT‐PCR). Results An immunohistochemistry study revealed that GC expressed PCSK 6 throughout follicular development, beginning in the primary follicle stage, while oocytes expressed PCSK 6 from the primordial follicle stage onwards. An in vitro study demonstrated that BMP‐2, BMP‐6, BMP‐7, and BMP‐15, not activin‐A and GDF‐9, decreased PCSK 6 gene expression in human GC. FSH induced PCSK 6 mRNA in the presence of activin‐A or GDF‐9. GDF‐3, which is an inhibitor of BMP cytokines, also induced PCSK 6 mRNA expression. Conclusions PCSK 6, which is a critical factor to produce BMP cytokines, was suppressed with BMP stimulation in human GC , suggesting the presence of a negative feedback system in the follicular development process.
Type of Medium:
Online Resource
ISSN:
1046-7408
,
1600-0897
DOI:
10.1111/aji.2012.68.issue-6
Language:
English
Publisher:
Wiley
Publication Date:
2012
detail.hit.zdb_id:
2024667-5
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