In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 70, No. 8_Supplement ( 2010-04-15), p. 519-519
Abstract:
Objective: The destruction of the basement membrane is the first step of cancer cell invasion and metastasis. Type IV collagen is a major component of the basement membrane, and is composed of six genetically distinct α(IV) chains: α1(IV) to α6 (IV). The loss of α5/α6(IV) chains from the epithelial basement membrane at the early stage of cancer cell invasion has been reported in several types of cancers. However, the expression of α5/α6(IV) chains in extrahepatic bile duct carcinoma remains unclear. The expression status of α5/α6(IV) chains in extrahepatic bile duct carcinoma was examined. Methods: The expression of α(IV) chains, p53, and Ki-67 were examined by using immunohistochemical method in 36 surgical specimens of extrahepatic bile duct carcinoma. The relationship between clinicopathological parameters of 36 patients and the results of immunohistochemical staining was statistically examined using χ2 test or Fisher's exact test. The overall cancer-specific survival was estimated using the Kaplan-Meier method, and the log-rank test was used to evaluate the statistical significance of the differences. Results: In the normal bile duct mucosa of all examined specimens, both α1/α2(IV) and α5/α6(IV) chains were stained in the basement membrane of the surface columnar epithelium in a continuous linear pattern, while both α3(IV) and α4(IV) chains were not stained. The α1/α2(IV) and α5/α6(IV) chains were also expressed in the basement membrane of smooth muscle cells of the muscularis propria and large blood vessels. In extrahepatic bile duct carcinoma, α5/α6(IV) chains disappeared partially or completely around the tubular cancer cells in 25 of 36 specimens. All of tumors expressed α5/α6(IV) chains were also positive for α1/α2(IV) chains. The expression of α5/α6(IV) chains were significantly correlated to T classification and TNM staging, and inversely correlated to p53 expression. No relationship was observed between the expression of α5/α6(IV) chains and Ki-67 index. The patients with loss of α1/2(IV) and α5/6(IV) chains demonstrated significantly poorer prognosis than those with positive α1/2(IV) chains, irrespective of α5/6(IV) expression. Conclusion: The loss of α1/2(IV) and α5/α6(IV) chains might be a useful prognostic factor for extrahepatic bile duct carcinoma. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 519.
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM10-519
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2010
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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