In:
European Journal of Immunology, Wiley, Vol. 40, No. 9 ( 2010-09), p. 2409-2422
Abstract:
Th17 cells and Th1 cells coordinate to play a critical role in the formation of inflammatory bowel diseases. To examine how Th17 and Th1 cells are regulated at inflammatory sites, we used Th1‐dominant CD4 + CD45RB high T cell‐transferred RAG‐2 −/− and Th1/Th17‐mixed IL‐10 −/− mice. Interestingly, not only did colitic RAG‐2 −/− mice that were parabiosed with WT mice show significant amelioration of colitis, but amelioration of disease was also observed in those parabiosed with colitic IL‐10 −/− mice. To assess the interference between Th1 and Th17 colitogenic T cells, we co‐transferred colitogenic CD4 + T cells from the lamina propria (LP) of CD4 + CD45RB high T cell‐transferred RAG‐2 −/− mice and IL‐10 −/− mice into RAG‐2 −/− mice. Surprisingly, the co‐transferred RAG‐2 −/− mice showed a vast cellular infiltration of LP CD4 + T cells similar to that seen in RAG‐2 −/− mice re‐transferred with the cells from colitic RAG‐2 −/− mice alone, but the co‐transferred RAG‐2 −/− mice did not have the wasting symptoms, which are also absent in RAG‐2 −/− mice transferred with cells from colitic IL‐10 −/− mice alone. Furthermore, the percentages of Th1 and Th17 cells originating from IL‐10 −/− mice and those of Th1 cells originating from colitic RAG‐2 −/− mice were all significantly decreased in the co‐transferred mice as compared with the singly‐transferred paired RAG‐2 −/− mice, suggesting that Th1 and Th17 cells are in competition, and that their orchestration results in a merged clinical phenotype of the two types of murine colitis.
Type of Medium:
Online Resource
ISSN:
0014-2980
,
1521-4141
DOI:
10.1002/eji.201040379
Language:
English
Publisher:
Wiley
Publication Date:
2010
detail.hit.zdb_id:
1491907-2
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