In:
Veterinary Science Development, PAGEPress Publications, Vol. 1, No. 1 ( 2011-05-24), p. 5-
Abstract:
The detection and diagnosis of tuberculosis (TB) in food-producing animals is critical to human health. In this study we applied the loop-mediated isothermal amplification (LAMP) system to detect 〈 em 〉 Mycobacterium tuberculosis 〈 /em 〉 complex (MTC) directly in 57 cattle and six lechwe ( 〈 em 〉 Kobus leche kafuensis 〈 /em 〉 ) carcasses exhibiting lesions characteristic of TB. The samples were first subjected to Ziehl-Neelsen microscopy, followed by culture and LAMP assay. In addition, multiplex-PCR was used to determine the species involved. Of the samples from the cattle, 84.2% (95% confidence interval: 71.6-92.1) were found positive with Ziehl-Neelsen microscopy, 93.0% (95% confidence interval: 82.2-97.7) with culture, and 94.7% (95% confidence interval: 84.5-98.6) with the LAMP system while the 〈 em 〉 Kobus leche kafuensis 〈 /em 〉 samples were all positive for all techniques used. These results indicate that the LAMP system can be used to augment the detection and surveillance of TB in animals; hence can be a very useful tool in the veterinary field and in public health.
Type of Medium:
Online Resource
ISSN:
2038-9701
,
2038-9698
DOI:
10.4081/vsd.2011.2374
Language:
Unknown
Publisher:
PAGEPress Publications
Publication Date:
2011
detail.hit.zdb_id:
2627484-X
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