In:
Evidence-Based Complementary and Alternative Medicine, Hindawi Limited, Vol. 2017 ( 2017), p. 1-12
Abstract:
Chai-Qin-Cheng-Qi decoction (CQCQD) improves intestinal motility in acute pancreatitis (AP), but the mechanism(s) require elucidation. We investigated the effects of CQCQD and carbachol, a prokinetic agent, on colonic smooth muscle cells (SMCs) in L-arginine-induced necrotising AP model in rats. In treatment groups, intragastric CQCQD (20 g/kg, 2 hourly × 3 doses) or intraperitoneal carbachol (60 μ g/kg) was given 24 hours after induction of AP. Both CQCQD and carbachol decreased the severity of pancreatic and colonic histopathology (all P 〈 0.05 ). Both CQCQD and carbachol reduced serum intestinal fatty acid binding protein, vasoactive intestinal peptide, and substance P and increased motility levels. CQCQD upregulated SMC phospholipase C-beta 1 (PLC- β 1) mRNA and PLC protein (both P 〈 0.05 ), while both treatments upregulated protein kinase C-alpha (PKC- α ) mRNA and PKC protein and downregulated adenylate cyclase (AC) mRNA and protein compared with no treatment (all P 〈 0.05 ). Neither treatment significantly altered L-arginine-induced PKC- β 1 and PKC- ε mRNA reduction. Both treatments significantly increased fluorescence intensity of SMC intracellular calcium concentration [ C a 2 + ] i (3563.5 and 3046.9 versus 1086.9, both P 〈 0.01 ). These data suggest CQCQD and carbachol improve intestinal motility in AP by increasing [ C a 2 + ] i in colonic SMCs via upregulating PLC, PKC and downregulating AC.
Type of Medium:
Online Resource
ISSN:
1741-427X
,
1741-4288
DOI:
10.1155/2017/5864945
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2017
detail.hit.zdb_id:
2148302-4
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