In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 75, No. 15_Supplement ( 2015-08-01), p. 1565-1565
Abstract:
Esophageal squamous cell carcinoma (ESCC) is one of the most common malignant neoplasms worldwide, especially in China. Patients are often diagnosed at advanced stages with poor prognosis due to the absence of obvious early symptoms. Therefore, there is an urgent need to develop novel serological biomarkers for ESCC. Here, we performed a two-stage serum peptide profiling in 477 unique samples using weak cationic exchange magnetic beads coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The three significantly differentially expressed peaks were used to establish a genetic algorithm model for ESCC. The sensitivity and specificity were 97% and 95.92% in the training set, and 97.03% and 100% in the validation set, respectively. It was significantly better than the other well studied serum proteins such as squamous cell carcinoma antigen (SCC-Ag) and cytokeratin 19 fragment 21-1 (Cyfra 21-1). Notably, the diagnostic capability of this model was superior for early stage ESCC with a sensitivity of 96.94% and a specificity of 100%. Furthermore, the three diagnostic peptides were identified as fragments of alpha-2-HS-glycoprotein, thrombospondin-1, and fibrinogen alpha, respectively. The immunohistochemical staining assay showed that TSP1 was significantly overexpressed in tumor tissues and associated with lymph node metastasis and poor prognosis. Overall, we constructed a novel diagnostic model which will benefit the screening, early detection and diagnosis of ESCC. And the identified proteins and their derivative peptides will deepen our understanding of tumorigenesis and serve as potential serological biomarkers for ESCC. Note: This abstract was not presented at the meeting. Citation Format: Kun Jia, Wei Li, Feng Wang, Fang Liu, Xiaofei Liu, Yuanyuan Qiao, Yang Xu, Lanping Zhou, Yulin Sun, Qingwei Ma, Xiaohang Zhao. Magnetic bead-based serum peptidome profiling for identifying potential biomarkers in esophageal squamous cell carcinoma. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 1565. doi:10.1158/1538-7445.AM2015-1565
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM2015-1565
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2015
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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