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  • Hindawi Limited  (2)
  • Sun, Ying  (2)
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  • Hindawi Limited  (2)
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  • 1
    Online Resource
    Online Resource
    NLRP3 Inflammasome Simultaneously Involved in Autophagy and Phagocytosis of THP-1 Cells to Clear Aged Erythrocytes
    Hindawi Limited ; 2022
    In:  Journal of Immunology Research Vol. 2022 ( 2022-9-30), p. 1-24
    Details
    In: Journal of Immunology Research, Hindawi Limited, Vol. 2022 ( 2022-9-30), p. 1-24
    Abstract: Autophagy and phagocytosis are two important processes that capture and digest materials found in cellular interiors and exteriors, respectively. Aged red blood cells (RBCs) are cleared by phagocytes in vivo. We focused on determining whether autophagy occurs after phagocytes swallow sunset erythrocytes, and whether the degree of autophagy is related to scavenging ability of phagocytes to erythrocytes. In addition, the ability of NLR family pyrin domain containing protein 3 (NLRP3) inflammasome to regulate erythrocyte clearance by phagocytes and its association with autophagy-related protein 16-like protein 1 (ATG16L1) are confirmed. We constructed a stable and low-NLRP3 expression THP-1 cell line using CRISPR/Cas9 technology. The analysis of erythrocyte clearance and autophagy of THP-1 cells with low NLRP3 expression showed that autophagy changes together when THP-1 engulfs aged RBCs. The occurrence of autophagy was dominated by microtubule-associated protein 1A/1B-light chain 3- (LC3-) associated phagocytosis accompanied by canonical autophagy. A negative correlation exists between the clearance of RBCs by THP-1 cells and the degree of autophagy. Downregulating the expression of NLRP3 in THP-1 cells can simultaneously inhibit the scavenging ability of THP-1 to erythrocytes and the degree of autophagy. In addition, the autophagy inhibitor bafilomycin A1 (BafA1) can enhance the phagocytosis ability of THP-1 to erythrocytes and promote the NLRP3 activation in THP-1 cells, while the autophagy inducer rapamycin inhibits the phagocytosis ability of THP-1 to RBCs and downregulates the NLRP3 activation. Results showed that autophagy and phagocytosis may be dynamic balance processes that can provide sufficient nutrition and energy to cells. Choosing NLRP3 as a target may regulate the phagocytic ability and the degree of autophagy in the meantime. These findings may be a potential strategy for regulating the clearance rate of phagocytes to aged RBCs and the secretion of proinflammatory cytokines to ensure transfusion safety.
    Type of Medium: Online Resource
    ISSN: 2314-7156 , 2314-8861
    URL: Article
    DOI: 10.1155/2022/1481154
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2817541-4
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    Online Resource
  • 2
    Online Resource
    Online Resource
    Free Vibrations and Impact Resistance of a Functionally Graded Honeycomb Sandwich Plate
    Hindawi Limited ; 2021
    In:  Shock and Vibration Vol. 2021 ( 2021-11-20), p. 1-15
    Details
    In: Shock and Vibration, Hindawi Limited, Vol. 2021 ( 2021-11-20), p. 1-15
    Abstract: The functionally graded honeycomb has the characteristic of light weight, low density, high impact resistance, noise reduction, and energy absorption as a kind of new composite inhomogeneous materials. It has the advantages of both functionally graded materials and honeycombs. In this paper, a functionally graded honeycomb sandwich plate with functionally graded distributed along the thickness of the plate is constructed. The equivalent elastic parameters of the functionally graded honeycomb core are given. Based on Reddy’s higher-order shear deformation theory (HSDT) and Hamilton’s principle, the governing partial differential equation of motion is derived under four simply supported boundary conditions. The natural frequencies of the graded honeycomb sandwich plate are obtained by both the Navier method from the governing equation and the finite element model. The results obtained by the two methods are consistent. Based on this, the effects of parameters and graded on the natural frequencies of the functionally graded honeycomb sandwich plate are studied. Finally, the dynamic responses of the functionally graded honeycomb sandwich plate under low-speed impacts are studied. The results obtained in this paper will provide a theoretical basis for further study of the complex dynamics of functionally graded honeycomb structures.
    Type of Medium: Online Resource
    ISSN: 1875-9203 , 1070-9622
    URL: Article
    DOI: 10.1155/2021/8043368
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2070162-7
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