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  • Su, Bin  (19)
  • Chemistry/Pharmacy  (19)
  • 1
    Online Resource
    Online Resource
    Microtube Electrodes for Imaging the Electrochemiluminescence Layer and Deciphering the Reaction Mechanism
    Wiley ; 2021
    In:  Angewandte Chemie International Edition Vol. 60, No. 4 ( 2021-01-25), p. 2089-2093
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 60, No. 4 ( 2021-01-25), p. 2089-2093
    Abstract: Electrochemiluminescence (ECL) is a powerful transduction technique in biosensing and diagnostics, while mechanistic studies are still scarce. Herein we report the combined use of microtube electrode (MTE) and microscopy to measure the thickness of ECL layer (TEL) to decipher reaction mechanisms. For the classical system involving tris(2,2′‐bipyridyl)ruthenium and tri‐ n ‐propylamine, the ECL pattern generated at the MTE tends to change from ring to spot upon increasing the luminophore concentration, with the TEL varying from ca. 3.1 μm to 〉 4.5 μm. This variation is rationalized to arise from the contribution of the so‐called catalytic route. While using 2‐(dibutylamino)ethanol as the co‐reactant, the ECL pattern remains ring‐shaped and independent on the luminophore concentration. The TEL in this case is ca. 2.1 μm, implying that ECL generation is always surface‐confined. MTEs can thus act as optical rulers for measuring the TEL and providing insightful mechanistic information.
    Type of Medium: Online Resource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v60.4
    DOI: 10.1002/anie.202012340
    RVK:
    VA 2100
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2011836-3
    detail.hit.zdb_id: 123227-7
    Location Call Number Limitation Availability
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  • 2
    Online Resource
    Online Resource
    Spatially Selective Imaging of Cell–Matrix and Cell–Cell Junctions by Electrochemiluminescence
    Wiley ; 2021
    In:  Angewandte Chemie International Edition Vol. 60, No. 21 ( 2021-05-17), p. 11769-11773
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 60, No. 21 ( 2021-05-17), p. 11769-11773
    Abstract: Cell junctions are protein structures located at specific cell membrane domains that determine key processes in multicellular development. Here we report spatially selective imaging of cell junctions by electrochemiluminescence (ECL) microscopy. By regulating the concentrations of luminophore and/or co‐reactant, the thickness of ECL layer can be controlled to match with the spatial location of different cell junctions. At a low concentration of luminophore, ECL generation is confined to the electrode surface, thus revealing only cell–matrix adhesions at the bottom of cells. While at a high concentration of luminophore, the ECL layer can be remarkably extended by decreasing the co‐reactant concentration, thus allowing the sequential imaging of cell–matrix and cell–cell junctions at the bottom and near the apical surface of cells, respectively. This strategy not only provides new insights into the ECL mechanisms but also promises wide applications of ECL microscopy in bioimaging.
    Type of Medium: Online Resource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v60.21
    DOI: 10.1002/anie.202101467
    RVK:
    VA 2100
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2011836-3
    detail.hit.zdb_id: 123227-7
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    Online Resource
  • 3
    Online Resource
    Online Resource
    Microtube Electrodes for Imaging the Electrochemiluminescence Layer and Deciphering the Reaction Mechanism
    Wiley ; 2021
    In:  Angewandte Chemie Vol. 133, No. 4 ( 2021-01-25), p. 2117-2121
    Details
    In: Angewandte Chemie, Wiley, Vol. 133, No. 4 ( 2021-01-25), p. 2117-2121
    Abstract: Electrochemiluminescence (ECL) is a powerful transduction technique in biosensing and diagnostics, while mechanistic studies are still scarce. Herein we report the combined use of microtube electrode (MTE) and microscopy to measure the thickness of ECL layer (TEL) to decipher reaction mechanisms. For the classical system involving tris(2,2′‐bipyridyl)ruthenium and tri‐ n ‐propylamine, the ECL pattern generated at the MTE tends to change from ring to spot upon increasing the luminophore concentration, with the TEL varying from ca. 3.1 μm to 〉 4.5 μm. This variation is rationalized to arise from the contribution of the so‐called catalytic route. While using 2‐(dibutylamino)ethanol as the co‐reactant, the ECL pattern remains ring‐shaped and independent on the luminophore concentration. The TEL in this case is ca. 2.1 μm, implying that ECL generation is always surface‐confined. MTEs can thus act as optical rulers for measuring the TEL and providing insightful mechanistic information.
    Type of Medium: Online Resource
    ISSN: 0044-8249 , 1521-3757
    URL: Issue
    URL: Article
    DOI: 10.1002/ange.v133.4
    DOI: 10.1002/ange.202012340
    RVK:
    VA 2100
    RVK:
    VN 5020
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 505868-5
    detail.hit.zdb_id: 506609-8
    detail.hit.zdb_id: 514305-6
    detail.hit.zdb_id: 505872-7
    detail.hit.zdb_id: 1479266-7
    detail.hit.zdb_id: 505867-3
    detail.hit.zdb_id: 506259-7
    Location Call Number Limitation Availability
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  • 4
    Online Resource
    Online Resource
    Electrochemiluminescence Distance and Reactivity of Coreactants Determine the Sensitivity of Bead‐Based Immunoassays
    Wiley ; 2023
    In:  Angewandte Chemie International Edition Vol. 62, No. 16 ( 2023-04-11)
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 62, No. 16 ( 2023-04-11)
    Abstract: Herein we report the study of electrochemiluminescence (ECL) generation by tris(2,2′‐bipyridyl)ruthenium (Ru(bpy) 3 2+ ) and five tertiary amine coreactants. The ECL distance and lifetime of coreactant radical cations were measured by ECL self‐interference spectroscopy. And the reactivity of coreactants was quantitatively evaluated in terms of integrated ECL intensity. By statistical analysis of ECL images of single Ru(bpy) 3 2+ ‐labeled microbeads, we propose that ECL distance and reactivity of coreactant codetermine the emission intensity and thus the sensitivity of immunoassay. 2,2‐bis(hydroxymethyl)‐2,2′,2′′‐nitrilotriethanol (BIS‐TRIS) can well balance ECL distance‐reactivity trade‐off and enhance the sensitivity by 236 % compared with tri‐ n ‐propylamine (TPrA) in the bead‐based immunoassay of carcinoembryonic antigen. The study brings an insightful understanding of ECL generation in bead‐based immunoassay and a way of maximizing the analytical sensitivity from the aspect of coreactant.
    Type of Medium: Online Resource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v62.16
    DOI: 10.1002/anie.202216525
    RVK:
    VA 2100
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2011836-3
    detail.hit.zdb_id: 123227-7
    Location Call Number Limitation Availability
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  • 5
    Online Resource
    Online Resource
    Silica Nanochannel Membranes for Electrochemical Analysis and Molecular Sieving: A Comprehensive Review
    Informa UK Limited ; 2020
    In:  Critical Reviews in Analytical Chemistry Vol. 50, No. 5 ( 2020-09-02), p. 424-444
    Details
    In: Critical Reviews in Analytical Chemistry, Informa UK Limited, Vol. 50, No. 5 ( 2020-09-02), p. 424-444
    Type of Medium: Online Resource
    ISSN: 1040-8347 , 1547-6510
    URL: Article
    DOI: 10.1080/10408347.2019.1642735
    RVK:
    VG 2450
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2020
    detail.hit.zdb_id: 2030019-0
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  • 6
    Online Resource
    Online Resource
    Electrochemiluminescence Waveguide in Single Crystalline Molecular Wires
    Wiley ; 2020
    In:  Angewandte Chemie International Edition Vol. 59, No. 17 ( 2020-04-20), p. 6745-6749
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 59, No. 17 ( 2020-04-20), p. 6745-6749
    Abstract: Here we report the first observation of active waveguide of electrochemiluminescence (ECL) in single crystalline molecular wires self‐assembled from cyclometalated iridium(III) complexes, namely tris(1‐phenylisoquinoline‐ C 2 , N ) (Ir(piq) 3 ). Under dark conditions, the molecular wires deposited on the electrode surface can act as both ECL emitters and active waveguides. As revealed by ECL microscopy, they exhibit the typical characteristics of optical waveguides, transmitting ECL and generating much brighter ECL emission at their terminals. Moreover, self‐generated ECL can be confined inside the molecular wire and propagates along the longitudinal direction as far as ≈100 μm to the terminal out of touch with the electrode. Therefore, this one‐dimensional crystalline molecular wire‐based waveguide offers the opportunity to switch the electrochemically generated ECL to remote light emission in non‐conductive regions and is promising for contactless electrochemical analysis and study of (bio)chemical systems.
    Type of Medium: Online Resource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v59.17
    DOI: 10.1002/anie.201915984
    RVK:
    VA 2100
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2011836-3
    detail.hit.zdb_id: 123227-7
    Location Call Number Limitation Availability
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    Online Resource
  • 7
    Online Resource
    Online Resource
    Electrochemiluminescence Distance and Reactivity of Coreactants Determine the Sensitivity of Bead‐Based Immunoassays
    Wiley ; 2023
    In:  Angewandte Chemie Vol. 135, No. 16 ( 2023-04-11)
    Details
    In: Angewandte Chemie, Wiley, Vol. 135, No. 16 ( 2023-04-11)
    Abstract: Herein we report the study of electrochemiluminescence (ECL) generation by tris(2,2′‐bipyridyl)ruthenium (Ru(bpy) 3 2+ ) and five tertiary amine coreactants. The ECL distance and lifetime of coreactant radical cations were measured by ECL self‐interference spectroscopy. And the reactivity of coreactants was quantitatively evaluated in terms of integrated ECL intensity. By statistical analysis of ECL images of single Ru(bpy) 3 2+ ‐labeled microbeads, we propose that ECL distance and reactivity of coreactant codetermine the emission intensity and thus the sensitivity of immunoassay. 2,2‐bis(hydroxymethyl)‐2,2′,2′′‐nitrilotriethanol (BIS‐TRIS) can well balance ECL distance‐reactivity trade‐off and enhance the sensitivity by 236 % compared with tri‐ n ‐propylamine (TPrA) in the bead‐based immunoassay of carcinoembryonic antigen. The study brings an insightful understanding of ECL generation in bead‐based immunoassay and a way of maximizing the analytical sensitivity from the aspect of coreactant.
    Type of Medium: Online Resource
    ISSN: 0044-8249 , 1521-3757
    URL: Issue
    URL: Article
    DOI: 10.1002/ange.v135.16
    DOI: 10.1002/ange.202216525
    RVK:
    VA 2100
    RVK:
    VN 5020
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 505868-5
    detail.hit.zdb_id: 506609-8
    detail.hit.zdb_id: 514305-6
    detail.hit.zdb_id: 505872-7
    detail.hit.zdb_id: 1479266-7
    detail.hit.zdb_id: 505867-3
    detail.hit.zdb_id: 506259-7
    Location Call Number Limitation Availability
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    Online Resource
  • 8
    Online Resource
    Online Resource
    Electrochemiluminescence Waveguide in Single Crystalline Molecular Wires
    Wiley ; 2020
    In:  Angewandte Chemie Vol. 132, No. 17 ( 2020-04-20), p. 6811-6815
    Details
    In: Angewandte Chemie, Wiley, Vol. 132, No. 17 ( 2020-04-20), p. 6811-6815
    Abstract: Here we report the first observation of active waveguide of electrochemiluminescence (ECL) in single crystalline molecular wires self‐assembled from cyclometalated iridium(III) complexes, namely tris(1‐phenylisoquinoline‐ C 2 , N ) (Ir(piq) 3 ). Under dark conditions, the molecular wires deposited on the electrode surface can act as both ECL emitters and active waveguides. As revealed by ECL microscopy, they exhibit the typical characteristics of optical waveguides, transmitting ECL and generating much brighter ECL emission at their terminals. Moreover, self‐generated ECL can be confined inside the molecular wire and propagates along the longitudinal direction as far as ≈100 μm to the terminal out of touch with the electrode. Therefore, this one‐dimensional crystalline molecular wire‐based waveguide offers the opportunity to switch the electrochemically generated ECL to remote light emission in non‐conductive regions and is promising for contactless electrochemical analysis and study of (bio)chemical systems.
    Type of Medium: Online Resource
    ISSN: 0044-8249 , 1521-3757
    URL: Issue
    URL: Article
    DOI: 10.1002/ange.v132.17
    DOI: 10.1002/ange.201915984
    RVK:
    VA 2100
    RVK:
    VN 5020
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 505868-5
    detail.hit.zdb_id: 506609-8
    detail.hit.zdb_id: 514305-6
    detail.hit.zdb_id: 505872-7
    detail.hit.zdb_id: 1479266-7
    detail.hit.zdb_id: 505867-3
    detail.hit.zdb_id: 506259-7
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 9
    Online Resource
    Online Resource
    Spatially Selective Imaging of Cell–Matrix and Cell–Cell Junctions by Electrochemiluminescence
    Wiley ; 2021
    In:  Angewandte Chemie Vol. 133, No. 21 ( 2021-05-17), p. 11875-11879
    Details
    In: Angewandte Chemie, Wiley, Vol. 133, No. 21 ( 2021-05-17), p. 11875-11879
    Abstract: Cell junctions are protein structures located at specific cell membrane domains that determine key processes in multicellular development. Here we report spatially selective imaging of cell junctions by electrochemiluminescence (ECL) microscopy. By regulating the concentrations of luminophore and/or co‐reactant, the thickness of ECL layer can be controlled to match with the spatial location of different cell junctions. At a low concentration of luminophore, ECL generation is confined to the electrode surface, thus revealing only cell–matrix adhesions at the bottom of cells. While at a high concentration of luminophore, the ECL layer can be remarkably extended by decreasing the co‐reactant concentration, thus allowing the sequential imaging of cell–matrix and cell–cell junctions at the bottom and near the apical surface of cells, respectively. This strategy not only provides new insights into the ECL mechanisms but also promises wide applications of ECL microscopy in bioimaging.
    Type of Medium: Online Resource
    ISSN: 0044-8249 , 1521-3757
    URL: Issue
    URL: Article
    DOI: 10.1002/ange.v133.21
    DOI: 10.1002/ange.202101467
    RVK:
    VA 2100
    RVK:
    VN 5020
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 505868-5
    detail.hit.zdb_id: 506609-8
    detail.hit.zdb_id: 514305-6
    detail.hit.zdb_id: 505872-7
    detail.hit.zdb_id: 1479266-7
    detail.hit.zdb_id: 505867-3
    detail.hit.zdb_id: 506259-7
    Location Call Number Limitation Availability
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    Online Resource
  • 10
    Online Resource
    Online Resource
    Development, validation and pilot screening of an in vitro multi-cellular three-dimensional cancer spheroid assay for anti-cancer drug testing
    Elsevier BV ; 2013
    In:  Bioorganic & Medicinal Chemistry Vol. 21, No. 4 ( 2013-2), p. 922-931
    Details
    In: Bioorganic & Medicinal Chemistry, Elsevier BV, Vol. 21, No. 4 ( 2013-2), p. 922-931
    Type of Medium: Online Resource
    ISSN: 0968-0896
    URL: Article
    DOI: 10.1016/j.bmc.2012.12.007
    RVK:
    VA 2619
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2013
    detail.hit.zdb_id: 1501507-5
    SSG: 15,3
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