In:
Arhiv za bioloske nauke, National Library of Serbia, Vol. 65, No. 1 ( 2013), p. 33-42
Kurzfassung:
Injury and other pathological conditions induce a massive release of ATP and
ADP that initiate an immune response. Extracellular nucleotides are degraded
by ectonucleotidases: enzymes from E-NTPDase and E-NPP families sequentially
hydrolyze ATP and ADP to AMP, which is further hydrolyzed by
ecto-5?-nucleotidase to adenosine that exerts suppressive effects on immune
cells. We investigated the ectonucleotidase activities of peripheral
lymphocytes at different post-injury times after an unilateral brain injury
in the rat. Significant and dynamic changes in the lymphocytic
ectonucleotidase activities were obtained. ATP- and ADP-hydrolysis changes,
together with their calculated ratios, indicate the major contribution of
E-NTPDase 1 and its comparable upregulation between sham operation and
injury. AMP hydrolysis changes were more brain-injury specific, with a
longer-lasting lymphocytic response induced by cortical stab injury (CSI). In
summary, CSI and sham operation induce the upregulation of the whole enzyme
chain for adenine nucleotide hydrolysis in lymphocytes, suggesting an
important roles of ectonucleotidases in the course of recovery after brain
injury.
Materialart:
Online-Ressource
ISSN:
0354-4664
,
1821-4339
Sprache:
Englisch
Verlag:
National Library of Serbia
Publikationsdatum:
2013
ZDB Id:
2380842-1
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