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  • SAGE Publications  (12)
  • Song, Wei  (12)
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  • SAGE Publications  (12)
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  • 1
    Online Resource
    Online Resource
    SAGE Publications ; 2021
    In:  Proceedings of the Institution of Mechanical Engineers, Part A: Journal of Power and Energy Vol. 235, No. 6 ( 2021-09), p. 1315-1331
    In: Proceedings of the Institution of Mechanical Engineers, Part A: Journal of Power and Energy, SAGE Publications, Vol. 235, No. 6 ( 2021-09), p. 1315-1331
    Abstract: Pressure side (PS) cutback structure has been widely employed in modern turbine blade trailing edge (TE) cooling design. However, hot gas flows around lip of TE slot to generate an impingement to cutback, resulting in a rapid decay of film cooling effectiveness and hence thermal damage of entire TE. Proper angled-ejections upstream of TE can suppress the downstream effectiveness-decay. Therefore, in present work, five types of film-holes were designed to discuss the effect of exit-shape of film-hole on TE film cooling characteristics, inlet boundary layer profile, fluid-interactions, discharge behaviors and total pressure losses. The studied models included cylindrical-hole, common and laidback fan-shaped film-holes, and converging slot-holes with two different exit-to-inlet area ratios ( ARs). The solo cutback cooling was chosen as a reference. Thermal tests were conducted in a hot wind tunnel featuring a simplified TE test section by infrared thermal technique. Engine-similar mainstream-to-coolant temperature ratio of 2.0 was controlled. Comparisons of experimental results revealed that the converging slot-holes can obtain the highest film cooling effectiveness at TE region and overall cooling effectiveness at PS film-plate, but the slightest increase of total pressure losses. Flow measurements using Particle Image Velocimetry technique indicated the converging slot-holes can modify the inlet boundary layer profile, suppress effectively the impingement of mainstream and change the flow separation features at cutback. In addition, applying shaped-holes can effectively suppress the flow ingestion under low coolant amounts. However, relative to the exit-shape effect, the coolant amount plays a more important role on the aerodynamic and thermal characteristics of TE cooling.
    Type of Medium: Online Resource
    ISSN: 0957-6509 , 2041-2967
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2021
    detail.hit.zdb_id: 2024892-1
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  • 2
    Online Resource
    Online Resource
    SAGE Publications ; 2021
    In:  High Performance Polymers Vol. 33, No. 4 ( 2021-05), p. 429-439
    In: High Performance Polymers, SAGE Publications, Vol. 33, No. 4 ( 2021-05), p. 429-439
    Abstract: To improve the safety of lithium-ion batteries (LIBs), a polyether amide–silica (PEI-SiO 2 ) composite membrane was developed by the in situ hydrolysis of tetraethylorthosilicate (TEOS) and its subsequent self-assembly on the surface of PEI fibers. Because of the presence of the SiO 2 shell, the PEI-SiO 2 composite membrane exhibited good thermal stability at high temperatures. The composite membrane did not change its color and size after heating at 200°C for 1 h as well as exhibited excellent flame retardancy. Moreover, the membrane maintained its high porosity even after the introduction of shell layers. The electrolyte is completely absorbed in the membrane within 0.5 s. The electrolyte uptake was up to 625%, and the ionic conductivity was up to 1.9 mS/cm at room temperature. Compared to the polyolefin membrane and the pure PEI membrane, the PEI-SiO 2 composite membrane showed higher electrochemical stability, with an electrochemical window of up to 5.5 V. The battery assembled with the composite membrane showed excellent cycle stability, and the capacity retention rate was as high as 98.6% after 50 cycles. The LIBs based on the PEI-SiO 2 composite membrane exhibited safe operation and high electrochemical performance, thus highlighting the applicability of the composite membrane in high-power batteries.
    Type of Medium: Online Resource
    ISSN: 0954-0083 , 1361-6412
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2021
    detail.hit.zdb_id: 1483713-4
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  • 3
    In: Energy Exploration & Exploitation, SAGE Publications
    Abstract: The methane adsorption at room temperature in the interlayer of the kaolinite–methanol complex (Kln–Me) with different methanol content is investigated with grand canonical Monte Carlo (GCMC) simulation. The mechanism and structure of methanol intercalated kaolinite (Kln) is proposed, and the effect of methanol on methane adsorption by Kln–Me is discussed. The results indicate that the methanol adsorption in the Kln interlayer is mostly physical with non-bonded energy. The interlayer spacing ( d) of Kln–Me optimized by the DREIDING force field is in good agreement with the experimental data measured with X-ray diffraction. The configuration, adsorption properties, and adsorption isotherms are obtained for eight Kln–Me systems with different number (2–20) of methanol molecules in interlayer space. By comparing methane adsorption in the Kln–Me interlayer with different number of methanol molecules, we discover the complex interplay of factors influencing methane adsorption in the Kln–Me interlayer, especially the number of methanol molecules and free volume. It is found that the adsorption capacity of Kln can be enhanced by inserting methanol molecules into its interlayer. This analysis also underscores the GCMC simulation as a viable tool to calculate kaolinite/organic intercalation composites for potential applications.
    Type of Medium: Online Resource
    ISSN: 0144-5987 , 2048-4054
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
    detail.hit.zdb_id: 2026571-2
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  • 4
    Online Resource
    Online Resource
    SAGE Publications ; 2014
    In:  European Journal of Mass Spectrometry Vol. 20, No. 4 ( 2014-08), p. 317-325
    In: European Journal of Mass Spectrometry, SAGE Publications, Vol. 20, No. 4 ( 2014-08), p. 317-325
    Abstract: The fragmentation mechanisms of singly protonated Gly-Asp-Gly-Arg (GDGR) and Arg-Gly-Asp-Gly (RGDG) were investigated by mass spectrometry and theoretical methods. Both protonated molecules are fragmented mainly at the Asp–Gly amide bond C-terminal to Asp, as supported by quantum chemical calculations. Charge distributions of C and N atoms ( Q C + Q N ) on the amide bonds were collected when the ionizing proton was fixed at different nitrogen atoms along the backbone for each peptide. Compared with the neutral molecules, the total charges of C and N atoms ( Q C + Q N ) for the singly charged peptides tended to be negative when the proton was located at the backbone nitrogen atoms. A relatively larger value of Q C + Q N corresponds to a higher trend of fragmentation, which is consistent with the experimental relative abundances data that the predominant ions are y 2 for [GDGR + H] + and b 3 for [RGDG + H] + . Also, the anhydride mechanism driven by the C-terminal COOH for [RGDG + H] + was explored by a quantum–mechanical/molecular–mechanical method. Calculations indicate that the protonated peptide can be cleaved through an unusual charge-directed pathway by forming a salt bridge at the C-termini. The formation of the anhydride linkage is much more feasible since this process needs very little energy and is exothermic, though the subsequent nucleophilic attack on the Asp carbonyl carbon is more difficult. The combined experimental and theoretical methods substantiate the mobile proton model, which opens a way to analyze quantitatively the discrepant fragmentation of dissociated peptides in peptide/protein identification.
    Type of Medium: Online Resource
    ISSN: 1469-0667 , 1751-6838
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2014
    detail.hit.zdb_id: 2021540-X
    detail.hit.zdb_id: 2021340-2
    SSG: 11
    SSG: 12
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  • 5
    In: The American Journal of Sports Medicine, SAGE Publications, Vol. 51, No. 8 ( 2023-07), p. 2005-2017
    Abstract: Adipose-derived stem cell (ADSC) sheets have been shown to promote tendon-to-bone healing. However, conventional laboratory preparation methods for ADSC sheets are time-consuming and risky, which precludes their diverse clinical applications. Purpose: To explore the utility of off-the-shelf cryopreserved ADSC sheets (c-ADSC sheets) for rotator cuff tendon-to-bone healing. Study Design: Controlled laboratory study. Methods: The ADSC sheets were cryopreserved and thawed for live/dead double staining, TdT-mediated dUTP Nick-End Labeling (TUNEL) staining, scanning electron microscopy observation, and biomechanical testing. Clone formation, proliferative capacity, and multilineage differentiation of ADSCs within the c-ADSC sheets were assayed to explore the effect of cryopreservation on stem cell properties. A total of 67 rabbits were randomly divided into 4 groups: normal group (without supraspinatus tendon tears; n = 7), control group (repair alone; n = 20), fresh ADSC (f-ADSC) sheet group (repair; n = 20), and c-ADSC sheet group (repair; n = 20). Rabbit bilateral supraspinatus tendon tears were induced to establish a chronic rotator cuff tear model. Gross observation, micro–computed tomography analysis, histological or immunohistochemical tests, and biomechanical tests were conducted at 6 and 12 weeks after repair. Results: No significant impairment was seen in the cell viability, morphology, and mechanical properties of c-ADSC sheets when compared with f-ADSC sheets. The stem cell properties of ADSC sheets also were preserved by cryopreservation. At 6 and 12 weeks after the repair, the f-ADSC and c-ADSC sheet groups showed superior bone regeneration, higher histological scores, larger fibrocartilage areas, more mature collagen, and better biomechanical results compared with the control group. No obvious difference was seen between the f-ADSC and c-ADSC sheet groups in terms of bone regeneration, histological score, fibrocartilage formation, and biomechanical tests. Conclusion: c-ADSC sheets, an off-the-shelf scaffold with a high potential for clinical translational application, can effectively promote rotator cuff tendon-to-bone healing. Clinical Relevance: Programmed cryopreservation of ADSC sheets is an efficient off-the-shelf scaffold for rotator cuff tendon-to-bone healing.
    Type of Medium: Online Resource
    ISSN: 0363-5465 , 1552-3365
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2023
    detail.hit.zdb_id: 2063945-4
    SSG: 31
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  • 6
    Online Resource
    Online Resource
    SAGE Publications ; 2019
    In:  The American Journal of Sports Medicine Vol. 47, No. 13 ( 2019-11), p. 3247-3255
    In: The American Journal of Sports Medicine, SAGE Publications, Vol. 47, No. 13 ( 2019-11), p. 3247-3255
    Abstract: Fatty infiltration, inflammation, and apoptosis are common degenerative changes in patients with chronic rotator cuff tears that can lead to muscle atrophy and can even result in massive irreparable rotator cuff tears. Some data have demonstrated the proregenerative, anti-inflammatory, and anti-apoptotic properties of stem cell–derived exosomes in some orthopaedic disorders, but their effect on torn rotator cuff muscles has never been investigated. Purpose: To study the effect of exosomes isolated from human adipose-derived stem cells (ASCs-Exos) on muscle degeneration, regeneration, and biomechanical properties in a rat model of a massive rotator cuff tear (MRCT). Study Design: Controlled laboratory study. Methods: A bilateral supraspinatus and infraspinatus tenotomy was performed on rats to create an MRCT model. Forty-two rats were randomly assigned to 3 groups: the sham surgery group, the saline group (lesions treated with a saline injection), and the ASCs-Exos group (lesions treated with an ASCs-Exos injection). Wet muscle weight, fatty infiltration, inflammation, vascularization, regeneration, and biomechanical properties were evaluated at 8 and 16 weeks after surgery. Results: The results revealed that the ASCs-Exos treatment could prevent the atrophy, fatty infiltration, inflammation, and vascularization of muscles in the MRCT model ( P 〈 .001). Additionally, the myofiber regeneration and biomechanical properties of ASCs-Exos-treated rotator cuffs were significantly elevated compared with those in the saline-treated group ( P 〈 .001). Conclusion: This study demonstrates that ASCs-Exos can effectively decrease atrophy and degeneration and improve muscle regeneration and biomechanical properties in torn rotator cuff muscles. Clinical Relevance: ASCs-Exos can be used as a new cell-free approach to prevent the muscle degeneration associated with torn rotator cuffs and may be helpful to repair torn rotator cuffs. Nevertheless, further work needs to be done in a large animal model owing to the inherent regenerative potential possessed by rodents.
    Type of Medium: Online Resource
    ISSN: 0363-5465 , 1552-3365
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2019
    detail.hit.zdb_id: 2063945-4
    SSG: 31
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  • 7
    Online Resource
    Online Resource
    SAGE Publications ; 2020
    In:  The American Journal of Sports Medicine Vol. 48, No. 6 ( 2020-05), p. 1456-1464
    In: The American Journal of Sports Medicine, SAGE Publications, Vol. 48, No. 6 ( 2020-05), p. 1456-1464
    Abstract: Fatty infiltration and poor tendon-bone healing in chronic rotator cuff tears (RCTs) are associated with unsatisfactory prognosis. Adipose stem cell–derived exosomes (ASC-Exos), having multiple biological effects, can prevent muscle degeneration in acute RCTs. However, the effects of ASC-Exos on fatty infiltration and tendon-bone healing in chronic RCTs remain unknown. Purpose: To study the effects of ASC-Exos on fatty infiltration and tendon-bone healing in a chronic RCT rabbit model. Study Design: Controlled laboratory study. Methods: At week 0, we randomly allocated 35 rabbits to receive sham surgery (14 rabbits) or establish a bilateral RCT model (21 rabbits, detachment of the supraspinatus tendon). At week 6, a total of 7 rabbits received sham surgery, and 7 rabbits with RCT were sacrificed for fatty infiltration assay. The remaining 14 rabbits with bilateral RCTs were randomly assigned to a saline group (7 rabbits that received local saline injection and rotator cuff repair) or an ASC-Exos group (7 rabbits that received local ASC-Exos injection and rotator cuff repair). At week 18, all rabbits were sacrificed for histological examination and biomechanical testing. Results: At week 18, the ASC-Exos group showed significantly lower fatty infiltration (14.01% ± 2.85%) compared with the saline group (21.79% ± 3.07%) ( P 〈 .001), and no statistical difference compared with the time of repair (10.88% ± 2.64%) ( P = .127). For tendon-bone healing, the ASC-Exos group showed a higher histological score and more newly regenerated fibrocartilage at the repair site than did the saline group. Regarding biomechanical testing, the ASC-Exos group showed significantly higher ultimate load to failure, stiffness, and stress than the saline group. Conclusion: Local injection of ASC-Exos in chronic RCTs at the time of repair could prevent the progress of fatty infiltration, promote tendon-bone healing, and improve biomechanical properties. Clinical Relevance: ASC-Exos injection may be used as a cell-free adjunctive therapy to inhibit fatty infiltration and improve rotator cuff healing in the repair of chronic RCTs.
    Type of Medium: Online Resource
    ISSN: 0363-5465 , 1552-3365
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2020
    detail.hit.zdb_id: 2063945-4
    SSG: 31
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  • 8
    Online Resource
    Online Resource
    SAGE Publications ; 2018
    In:  Advances in Mechanical Engineering Vol. 10, No. 7 ( 2018-07), p. 168781401878737-
    In: Advances in Mechanical Engineering, SAGE Publications, Vol. 10, No. 7 ( 2018-07), p. 168781401878737-
    Abstract: Regular surface-maintenances are necessary for high structures to increase service life. The traditional manual operation has shortcomings like limited maneuverability, poor operating quality, low operating efficiency, and high risk of physical harm, which makes it urgent to develop wall-climbing robot for carrying out surface-maintenances of high structures with high efficiency, low cost, and good protection of operators. In this article, we have developed a wheeled wall-climbing robot that uses a permanent magnet adhesion system to climb on large steel surfaces. Wheel traction to avoid slippage is increased by using inflated rubber tire while maintaining a desired air gap for the magnet system. Research is directed at designing a lightweight magnet system to provide an optimum adhesion force and at determining required tire pressures to maintain a specified air gap between the magnets and the surface.
    Type of Medium: Online Resource
    ISSN: 1687-8140 , 1687-8140
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2018
    detail.hit.zdb_id: 2501620-9
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  • 9
    Online Resource
    Online Resource
    SAGE Publications ; 2015
    In:  Proceedings of the Institution of Mechanical Engineers, Part B: Journal of Engineering Manufacture Vol. 229, No. 12 ( 2015-12), p. 2284-2300
    In: Proceedings of the Institution of Mechanical Engineers, Part B: Journal of Engineering Manufacture, SAGE Publications, Vol. 229, No. 12 ( 2015-12), p. 2284-2300
    Abstract: Designers often have some difficulties in fulfilling complex product design due to the lack of design resources. It is a crucial issue that how to help designers to produce design from enormous, isomeric, and even distributed design resources of virtual prototyping and then ally those correlative distributed design resources to collaborative design. This article is devoted to an object-oriented design resource management for virtual prototyping in collaborative design. After the design resource unit model is proposed with the representation model of virtual prototyping, the object-oriented product design resource representation framework for virtual prototyping is then put forward. The storage, usage, search, version management, and security management strategies for design resources of virtual prototyping are then discussed in detail. The framework of design resource management system for virtual prototyping is also put forward to support collaborative design. The collaborative design of cold heading machine is given as an example, which demonstrates that the methodology is helpful to distributed knowledge reuse for collaborative design.
    Type of Medium: Online Resource
    ISSN: 0954-4054 , 2041-2975
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2015
    detail.hit.zdb_id: 2032745-6
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  • 10
    In: Experimental Biology and Medicine, SAGE Publications, Vol. 238, No. 8 ( 2013-08), p. 951-959
    Abstract: Stem cell therapy may provide a therapeutic method for the replacement and regeneration of damaged neurons of the central nervous system. However, neural stem cells (NSCs) and neural precursor cells (NPCs) are especially vulnerable after transplantation due to a lack of sufficient growth factors at the transplant site. Electrical stimulation (ES) has recently been found to participate in the regulation of cell proliferation, growth, differentiation, and migration, but its underlying anti-apoptotic effects remain unclear. This study investigated the protective effects of biphasic electrical stimulation (BES) on olfactory bulb NPCs against growth factor-deprived apoptosis, examining the survival and apoptotic features of the cells. Differentiation was assessed by neuronal and glial markers. Brain-derived neurotrophic factor–phosphatidylinositol 3′-kinase (BDNF)-PI3K/Akt pathway activation was determined by enzyme-linked immunosorbent assay and Western blot. The chemical inhibitor wortmannin was used to inhibit the PI3K/Akt pathway. BES exerts a protective effect against growth factor-deprived apoptosis in the NPCs. BES enhanced cell survival and decreased the apoptotic/necrotic rate. Expression of phosphorylated Akt and BDNF secretion increased with BES for 12 h. Furthermore, the protective effects of BES were inhibited by blocking PI3K/AKT signalling. These results suggest that BES prevents growth factor-deprived apoptosis through the BDNF-PI3K/Akt signalling. This work strengthens the opinion that BES may be used as an auxiliary strategy for improving cell survival and preventing cell apoptosis in stem cell-based transplantation therapy.
    Type of Medium: Online Resource
    ISSN: 1535-3702 , 1535-3699
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2013
    detail.hit.zdb_id: 2020856-X
    SSG: 12
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