In:
Molecular Systems Biology, EMBO, Vol. 13, No. 2 ( 2017-02)
Abstract:
image Recombinase Directed Indexing (REDI), a new method that facilitates parsing and purification of array‐synthesized oligonucleotide pools, is presented. REDI is applied to produce a high‐quality DNA probe library and an arrayed collection of CRISPRi strains for essential yeast genes. A protocol and requisite strain resources are developed for Recombinase Directed Indexing (REDI), a method enabling parsing and purifying complex DNA libraries in yeast. The method is used to produce a highly uniform, sequence‐verified pool of ˜3,300 oligonucleotide probes for bacterial detection. The method is also used to create an arrayed collection of ˜9,000 CRISPR interference strains for conditional repression of yeast genes essential for fermentative or respiratory growth.
Type of Medium:
Online Resource
ISSN:
1744-4292
,
1744-4292
DOI:
10.15252/msb.20167233
Language:
English
Publisher:
EMBO
Publication Date:
2017
detail.hit.zdb_id:
2193510-5
SSG:
12
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