In:
FEBS Letters, Wiley, Vol. 321, No. 2-3 ( 1993-04-26), p. 224-228
Abstract:
Activity of protein phosphatase measured in the absence of divalent cations was decreased by 50% during all‐trans retinoic acid (ATRA)‐induced HL‐60 cell differentiation into the granulocytic phenotype. Treatment of HL‐60 cells with ATRA led to a dramatic decrease in the amount of protein phosphatase type 2A (PP2A) protein, whereas that of protein phosphatase type l (PP1) protein was relatively constant, as detected by immunoblotting with antibodies specific to PP1 and PP2A. The decreased phosphatase activity may be mainly due to a decrease in the expression of the PP2A protein. The mRNA level ofPP2Aβ was markedly decreased within 5 h after addition of ATRA, but there was only a slight increase in the mRNA level of PP2Aα. Selective down‐regulation of PP2Aβ mRNA clearly preceded the cell differentiation induced by ATRA treatment. Thus, PP2A is down‐regulated during ATRA‐induced differentiation of HL‐60 cells into granulocytes.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(93)80113-9
Language:
English
Publisher:
Wiley
Publication Date:
1993
detail.hit.zdb_id:
1460391-3
SSG:
12
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