In:
Oncology, S. Karger AG, Vol. 79, No. 3-4 ( 2010), p. 229-237
Abstract:
〈 i 〉 Objective: 〈 /i 〉 To clarify the role of DNA polymerase delta in tumor progression, we examined the expression of its main catalytic subunit p125 encoded by 〈 i 〉 POLD1 〈 /i 〉 in hepatocellular carcinoma (HCC) and human HCC cell lines. 〈 i 〉 Methods: 〈 /i 〉 We examined the expression of p53 and p125 in HCC by using immunohistochemistry and Western blotting. Characteristic changes observed in human HCC cell lines after transfection were examined. 〈 i 〉 Results: 〈 /i 〉 Immunohistochemical examination revealed positive staining of p125 in HCC cell nuclei, but few positively stained cells were observed in noncancerous tissues (p 〈 0.0001). p125 expression in specimens significantly correlated with cellular differentiation (p = 0.0048) and the degree of vascular invasion (p = 0.0401). It also significantly correlated with abnormal p53 expression. In vivo studies showed that p125 was upregulated in mutant p53-transfected HepG2 cells, which had more invasive potential than did control cells. Furthermore, the expression and invasive potential were reduced by the silencer sequence for 〈 i 〉 POLD1 〈 /i 〉 . 〈 i 〉 Conclusions: 〈 /i 〉 These findings suggest that the DNA polymerase delta catalytic subunit p125 induced by mutant type p53 plays an important role in tumor invasion, which leads to a poorer prognosis in HCC.
Type of Medium:
Online Resource
ISSN:
0030-2414
,
1423-0232
Language:
English
Publisher:
S. Karger AG
Publication Date:
2010
detail.hit.zdb_id:
1483096-6
detail.hit.zdb_id:
250101-6
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