In:
Microbiology and Immunology, Wiley, Vol. 57, No. 11 ( 2013-11), p. 762-771
Abstract:
The protective efficacy of and immune response to heat‐killed cells of monovalent and hexavalent mixtures of six serogroups/serotypes of Shigella strains ( Shigella dysenteriae 1, Shigella flexneri 2a, S. flexneri 3a, S. flexneri 6, Shigella boydii 4, and Shigella sonnei ) were examined in a guinea pig colitis model. A monovalent or hexavalent mixture containing 1 × 10 7 of each serogroup/serotype of heat‐killed Shigella cells was administered orally on Days 0, 7, 14 and 21. On Day 28, the immunized animals were challenged rectally with 1 × 10 9 live virulent cells of each of the six Shigella serogroups/serotypes. In all immunized groups, significant levels of protection were observed after these challenges. The serum titers of IgG and IgA against the lipopolysaccharide of each of the six Shigella serogroups/serotypes increased exponential during the course of immunization. High IgA titers against the lipopolysaccharide of each of the six Shigella serogroups/serotypes were also observed in intestinal lavage fluid from all immunized animals. These data indicate that a hexavalent mixture of heat‐killed cells of the six Shigella serogroups/serotypes studied would be a possible broad‐spectrum candidate vaccine against shigellosis.
Type of Medium:
Online Resource
ISSN:
0385-5600
,
1348-0421
DOI:
10.1111/1348-0421.12095
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
224792-6
detail.hit.zdb_id:
2102145-4
SSG:
12
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