In:
Digestion, S. Karger AG, Vol. 74, No. 3-4 ( 2006), p. 187-198
Abstract:
〈 i 〉 Background/Aim: 〈 /i 〉 The aim of this study was to see whether administration of celecoxib, a selective COX-2 inhibitor, prior to the appearance of intestinal metaplasia could prevent the development of gastric cancer in 〈 i 〉 Helicobacter pylori 〈 /i 〉 -infected Mongolian gerbils. 〈 i 〉 Methods: 〈 /i 〉 Fifty-two Mongolian gerbilswere divided into 3 groups and given 5 biweekly doses of N-methyl-N-nitrosourea (MNU; 30 ppm). At week 12, group 2 (n = 20) and group 3 (n = 22) gerbils were then given an injection of 〈 i 〉 H. pylori 〈 /i 〉 , while group 1 controls (n = 10) received 〈 i 〉 Brucella 〈 /i 〉 broth alone. In addition, 7 weeks after 〈 i 〉 H. pylori 〈 /i 〉 inoculation, at week 19, group 3 gerbils also received a 36-week administration course of celecoxib (1,500 ppm) in their diet. The incidence of gastric adenocarcinoma was determined at week 54 by histological analysis. COX-2 and Cdx2 protein expression and COX activity were evaluated for each group. The extent of intestinal metaplasia, Cdx2 and MUC2 expression, and the apoptotic index were evaluated semi-quantitatively by immunohistochemistry. 〈 i 〉 Results: 〈 /i 〉 The incidence of gastric adenocarcinoma was: group 1, 0% (0/10); group 2, 65% (13/20), and group 3, 23% (5/22; p 〈 0.05). Continuous celecoxib administration significantly reduced COX activity and COX-2 protein expression, Cdx2 and MUC2 protein immunoreactivity, and the extent of Alcian blue periodic acid-Schiff-positive intestinal metaplasia in 〈 i 〉 H. pylori 〈 /i 〉 -infected gerbils. Celecoxib also induced apoptosis in these gerbils. Significant inhibition of Cdx2 expression in group 3 gerbils was also shown by Western blot analysis. 〈 i 〉 Conclusions: 〈 /i 〉 Prior to the first appearance of intestinal metaplasia, timely administration of celecoxib prevents gastric cancer occurrence by disrupting the progression of intestinal metaplasia into gastric carcinoma through its inhibition of Cdx2 expression in MNU-pretreated 〈 i 〉 H. pylori 〈 /i 〉 -infected Mongolian gerbils.
Type of Medium:
Online Resource
ISSN:
0012-2823
,
1421-9867
Language:
English
Publisher:
S. Karger AG
Publication Date:
2006
detail.hit.zdb_id:
1482218-0
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