In:
ECS Transactions, The Electrochemical Society, Vol. 3, No. 10 ( 2006-10-20), p. 81-86
Abstract:
We have fabricated a precisely controlled nano-gapped electrode consisting of a nanoparticle - alkylchain - nanoparticle repeated sequence with a self-assembly process of Au and alkylthiol. The electrode was applied to the label-free DNA detection enabling us to read out the response purely by electronic protocols. After an addition of target DNA to the probe modified film, the resistance of the film dropped rapidly and then became constant within 30 s. The resistance reduction is attributed to the conductivity of the hybridized DNA, which can be explained by the dense π overlapping of adjacent base pairs in the double helix forming between the Au particles. The resistance changes depended on the number of mismatching bases in the DNA. Moreover, a significant difference in the resistance change was observed between complementary and 1 base pair mismatching DNA, which suggests that the film is characterized as an important diagnostic advantage on detecting single nucleotide polymorphism.
Type of Medium:
Online Resource
ISSN:
1938-5862
,
1938-6737
Language:
Unknown
Publisher:
The Electrochemical Society
Publication Date:
2006
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