In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 2 ( 2021-2-19), p. e0246125-
Abstract:
Drug-induced allergy (DIA), an unexpectedly triggered side effect of drugs used for therapeutic purposes, is a serious clinical issue that needs to be resolved because it interrupts the treatment of the primary disease. Since conventional allergy testing is insufficient to accurately predict the occurrence of DIA or to determine the drugs causing it, the development of diagnostic and predictive tools for allergic reactions is important. We demonstrated a novel method, termed high-sensitive allergy test (HiSAT), for the rapid diagnosis of allergy (within 1 hr; with true-positive diagnosis rates of 89% and 9% for patients with and without allergy-like symptoms, respectively). HiSAT analyzes the cell kinetics as an index against chemotactic factors in a patient’s serum, as different from the diagnosis using conventional methods. Once allergy has occurred, HiSAT can be used to determine the causative medicine using culture supernatants incubated with the subject’s lymphocytes and the test allergen. This test is more efficient (60%) than the lymphocyte transformation test (20%). Furthermore, in HiSAT, cell mobility significantly increases in a dose-dependent manner against supernatant incubated with lymphocytes from a subject with pollinosis collected at a time when the subject is without allergic symptoms and the antigen. The result demonstraed that HiSAT might be a promising method to rapidly diagnose DIA or to determine with high accuracy the antigen causing allergy.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0246125
DOI:
10.1371/journal.pone.0246125.g001
DOI:
10.1371/journal.pone.0246125.g002
DOI:
10.1371/journal.pone.0246125.g003
DOI:
10.1371/journal.pone.0246125.g004
DOI:
10.1371/journal.pone.0246125.g005
DOI:
10.1371/journal.pone.0246125.g006
DOI:
10.1371/journal.pone.0246125.t001
DOI:
10.1371/journal.pone.0246125.t002
DOI:
10.1371/journal.pone.0246125.s001
DOI:
10.1371/journal.pone.0246125.s002
DOI:
10.1371/journal.pone.0246125.s003
DOI:
10.1371/journal.pone.0246125.s004
DOI:
10.1371/journal.pone.0246125.s005
DOI:
10.1371/journal.pone.0246125.s006
DOI:
10.1371/journal.pone.0246125.s007
DOI:
10.1371/journal.pone.0246125.s008
DOI:
10.1371/journal.pone.0246125.s009
DOI:
10.1371/journal.pone.0246125.s010
DOI:
10.1371/journal.pone.0246125.s011
DOI:
10.1371/journal.pone.0246125.s012
DOI:
10.1371/journal.pone.0246125.s013
DOI:
10.1371/journal.pone.0246125.s014
DOI:
10.1371/journal.pone.0246125.s015
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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