In:
The Analyst, Royal Society of Chemistry (RSC), Vol. 147, No. 23 ( 2022), p. 5419-5427
Abstract:
Developing carbon-based materials with high catalytic performance and sensitivity has significance in low-cost and highly efficient nanozymes. Herein, for the first time, Cu,N-codoped hollow carbon nanospheres (CuNHCNs) with highly active Cu-Nx sites were successfully assembled through a template-free strategy, in which Cu 2+ -poly( m -phenylenediamine) (Cu-PmPD) nanospheres were utilized as the source of Cu, N and C. Benefiting from the synergistic effect of the hollow spherical structure and optimized composition, the CuNHCN exhibits high affinity for 3,3′,5,5′-tetramethylbenzidine and H 2 O 2 with 0.0655 mM and 0.918 mM, respectively, which are superior to those of HRP and most metal-based nanozymes. Moreover, by employing glucose and ascorbic acid (AA) as biomolecule models, a CuNHCN-based colorimetric detection platform is developed. The CuNHCN exhibits superior peroxidase mimicking activity and sensitivity in detecting glucose and AA with a detection limit of 0.187 μM and 68.9 nM (S/N = 3), respectively. Also, the colorimetric detection based on the CuNHCN towards glucose and AA in human serum presents superior practicability and accuracy. The assay provides a new avenue for designing and fabricating low-cost peroxidase nanozymes with high performance in bioassays.
Type of Medium:
Online Resource
ISSN:
0003-2654
,
1364-5528
Language:
English
Publisher:
Royal Society of Chemistry (RSC)
Publication Date:
2022
detail.hit.zdb_id:
1472713-4
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