GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    Kualitas dan Tingkat Maturasi Oosit Kucing Domestik dari Ovarium yang Disimpan dalam Waktu dan Media yang Berbeda
    Jurnal Veteriner ; 2021
    In:  Jurnal Veteriner Vol. 22, No. 3 ( 2021-09-30), p. 374-382
    Details
    In: Jurnal Veteriner, Jurnal Veteriner, Vol. 22, No. 3 ( 2021-09-30), p. 374-382
    Abstract: Penelitian ini bertujuan untuk mengevaluasi kualitas dan tingkat maturasi oosit kucing domestik yang disimpan dalam waktu dan media yang berbeda. Ovarium yang diperoleh setelah ovaryohisterectomy disimpan dalam tabung steril dan cara penyimpanannya dibagi menjadi tiga perlakuan , yaitu: 1) tanpa media, 2) berisi NaCl 0,9% atau 3) berisi PBS. Ovarium tersebut kemudian dibawa ke laboratorium dengan termos yang berisi NaCl 0,9% dengan suhu 35-37°C atau dengan cooler box suhu 4°C. Sampel ovarium suhu 4°C kemudian disimpan dalam refrigerator dengan suhu 4°C selama 24 dan 48 jam. Oosit dari ovarium yang dibawa dengan suhu suhu 35-37°C dikoleksi dalam waktu di bawah enam jam setelah sampai di laboratorium. Pada akhir penyimpanan, oosit dikoleksi dan dievaluasi kualitasnya. Selanjutnya, oosit dimaturasi dan dievaluasi tingkat maturasinya. Hasil dari penelitian ini menunjukkan bahwa berdasarkan morfologinya kualitas oosit kucing tidak dipengaruhi oleh waktu dan jenis media selama penyimpanan (P 〉 0,05). Tingkat maturasi oosit untuk mencapai tahap MII mulai menurun (P 〈 0,05) pada ovarium yang disimpan tanpa media maupun dengan PBS pada 24 jam periode penyimpanan, sedangkan oosit yang berasal dari ovarium yang disimpan dengan NaCl 0,9% mulai menurun (P 〈 0,05) pada 48 jam periode penyimpanan. Simpulan pada penelitian ini adalah penyimpanan ovarium dengan atau tanpa media selama 48 jam tidak memengaruhi morfologi oosit kucing namun memengaruhi tingkat maturasi oosit kucing.
    Type of Medium: Online Resource
    ISSN: 1411-8327 , 2477-5665
    Uniform Title: QUALITY AND MATURATION RATE OF DOMESTIC CAT OOCYTE FROM OVARY STORED IN DIFFERENT STORAGE TIME AND MEDIUM
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.19087/jveteriner.2021.22.3
    DOI: 10.19087/jveteriner
    DOI: 10.19087/jveteriner.2021.22.3.374
    Language: Unknown
    Publisher: Jurnal Veteriner
    Publication Date: 2021
    detail.hit.zdb_id: 2911720-3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Adiponectin: Potential Protein Hormone as a Candidate Biomarker for Male Fertility
    Indonesian Center for Animal Research and Development (ICARD) ; 2019
    In:  Indonesian Bulletin of Animal and Veterinary Sciences Vol. 29, No. 4 ( 2019-12-07), p. 183-
    Details
    In: Indonesian Bulletin of Animal and Veterinary Sciences, Indonesian Center for Animal Research and Development (ICARD), Vol. 29, No. 4 ( 2019-12-07), p. 183-
    Abstract: Reproduction is an important factor that requires attention to increase livestock production. The application of artificial insemination (AI) technology has been developed rapidly in the world, so that availability of fertile superior male is a determinant factor of successful AI. Several recent studies have been aimed to discover male fertility biomarkers, by intense research on fertility-assosiated proteins contained in seminal plasma. This paper describes the role of adiponectin as a candidate of male biomarker fertility. Adiponectin has a positive effect on spermatozoa functionality and steroidogenesis. This has been proven by several studies that found its expression in Sertoli cells and Leydig cells in the reproductive tract. Based on the specific role on spermatozoa functionality and steroidogenesis in supporting male fertility parameter, it is strongly suggested that adiponectin is an excellent candidate biomarker for male fertility.
    Type of Medium: Online Resource
    ISSN: 2354-6832 , 0216-6461
    URL: Article
    DOI: 10.14334/wartazoa.v29i4.2063
    Language: Unknown
    Publisher: Indonesian Center for Animal Research and Development (ICARD)
    Publication Date: 2019
    detail.hit.zdb_id: 2853339-2
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    TINGKAT PERKEMBANGAN AWAL EMBRIO SAPI IN VITRO MENGGUNAKAN MEDIA TUNGGAL BERBAHAN DASAR TISSUE CULTURE MEDIUM (TCM) 199
    LPPM Unsyiah ; 2013
    In:  Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Vol. 7, No. 2 ( 2013-09-01)
    Details
    In: Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences, LPPM Unsyiah, Vol. 7, No. 2 ( 2013-09-01)
    Abstract: Penelitian ini bertujuan mengetahui kemampuan perkembangan awal embrio sapi in vitro menggunakan media tunggal untuk maturasi, fertilisasi, dan kultur berbahan dasar tissue culture medium (TCM) 199. Oosit sapi dikumpulkan dari rumah potong hewan dengan teknik aspirasi dan diklasifikasikan berdasarkan kekompakan sel kumulus dan sitoplasma yang homogen. Oosit dimaturasi pada medium TCM 199 yang disuplementasi dengan 10 IU/ml pregnant mare’s serum gonadotropin (PMSG), 10 IU/ml human chorionic gonadotropin (hCG), dan 10% fetal bovine serum (FBS), dilakukan selama 24 jam pada inkubator 5% CO2, 39 C. Fertilisasi dilakukan pada dua media yang berbeda yaitu media rutin fertilisasi dan media berbahan dasar TCM 199 dengan suplemen bovine serum albumin (BSA) dan heparin. Setelah fertilisasi, kumulus sel dihilangkan (denudasi), kemudian dikultur pada media TCM 199 yang disuplementasi dengan asam amino esensial dan non-esensial serta 10% FBS selama 3 hari. Hasil penelitian menunjukkan tingkat maturasi oosit pada sistem yang digunakan mampu mendukung 81,5% oosit mencapai tahap metafase II (M-II). Tingkat pembelahan embrio lebih tinggi pada media rutin dibandingkan dengan media TCM 199 yakni masing-masing 44,4 dan 23,2%. Jumlah embrio tahap 4-8 sel pada kedua perlakuan tidak berbeda nyata. Dapat disimpulkan media tunggal berbasis TCM dapat digunakan untuk produksi embrio in vitro.
    Type of Medium: Online Resource
    ISSN: 2502-5600 , 1978-225X
    URL: Article
    DOI: 10.21157/j.ked.hewan.v7i2.930
    Language: Unknown
    Publisher: LPPM Unsyiah
    Publication Date: 2013
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    PRODUCTIVITY AND FRESH SEMEN CHARACTERISTICS OF SIMMENTAL BULL DIFFERENT AGES
    LPPM Unsyiah ; 2022
    In:  Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Vol. 16, No. 1 ( 2022-04-14), p. 23-28
    Details
    In: Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences, LPPM Unsyiah, Vol. 16, No. 1 ( 2022-04-14), p. 23-28
    Abstract: This study aimed to determine the effect of age on bulls’ productivity and fresh semen characteristics of Simmental bull in Indonesia. A total of 1071 data of semen collection and production from four age groups (two years old (yo), four yo, ≥ 10 yo with high semen rejection (≥10 HR), and ≥ 10 yo with low semen rejection (≥10 LR) were used in this study to evaluate the productivity and characteristics of fresh semen. The results showed that the pre-freezing and post-freezing semen rejection rate of ≥10 HR group was higher (P0.05) than the other groups. The four yo group had the percentage of second semen ejaculation each collection was higher (P0.05) than the other groups. Furthermore, semen volume, sperm concentration, and total sperm concentration significantly increased (P0.05) until four yo and then decreased (P0.05) in ≥ 10 yo groups. The ≥10 HR group had the volume and total sperm concentration significantly different (P0.05) with a group of ≥10 LR. Total sperm motility, individual motility, and mass movement were lower (P0.05) in ≥10 HR than the other groups. In conclusion, age differences of bulls can affect the productivity and characteristics of fresh semen.
    Type of Medium: Online Resource
    ISSN: 2502-5600 , 1978-225X
    URL: Article
    DOI: 10.21157/j.ked.hewan.v16i1.23487
    Language: Unknown
    Publisher: LPPM Unsyiah
    Publication Date: 2022
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    THE EFFECTIVITY OF CYSTEAMINE SUPPLEMENTATION ON IMPROVING THE IN VITRO FERTILIZATION RATE OF SHEEP OOCYTES
    LPPM Unsyiah ; 2021
    In:  Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Vol. 15, No. 4 ( 2021-12-31), p. 118-122
    Details
    In: Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences, LPPM Unsyiah, Vol. 15, No. 4 ( 2021-12-31), p. 118-122
    Abstract: The purpose of this study was to evaluate the effectiveness of cysteamine supplementation on the maturation medium and/or in vitro fertilization medium with regards to improving the normal fertilization rate of sheep oocytes, which are characterized by the formation of two pronuclei. Grade A and B oocytes were matured in medium-199 with 0.3% bovine serum albumin (BSA), 10 IU/mL follicle stimulating hormone (FSH), 10 IU/mL human chorionic gonadotrophin (hCG), and 50 μg/mL gentamicin added for 24 hours in a 5% CO2 incubator at 39° C. The treatment group was divided into the following groups: a control group with no cysteamine (P1), a group with 100 μM cysteamine in the maturation medium (P2), a group with 100 μM cysteamine in the fertilization medium (P3), and a group with 100 μM cysteamine both in the maturation and fertilization medium (P4). The fertilization was carried out by incubating sperm-oocytes for 12 hours and then staining them with 1% aceto-orcein to observe the formation of a pronucleus. Normal fertilization rates obtained by each treatment group were 56.5% (P1), 57.1% (P2), 57.8% (P3), and 59.9% (P4) with no significant difference (P0.05) between groups. It was concluded that 100 μM cysteamine supplementation in both the maturation medium and fertilization medium was not able to increase the normal fertilization rate of sheep oocytes.
    Type of Medium: Online Resource
    ISSN: 2502-5600 , 1978-225X
    URL: Article
    DOI: 10.21157/j.ked.hewan.v15i4.17688
    Language: Unknown
    Publisher: LPPM Unsyiah
    Publication Date: 2021
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 6
    Online Resource
    Online Resource
    Status DNA dan Karakteristik Spermatozoa Kauda Epididimis Domba Pascapenyimpanan pada Suhu 4oC (DNA STATUS AND CHARACTERISTIC OF SPERM CAUDA EPIDIDYMAL RAM AFTER STORAGE AT 4oC)
    Jurnal Veteriner ; 2017
    In:  Jurnal Veteriner Vol. 18, No. 2 ( 2017-06-20), p. 167-
    Details
    In: Jurnal Veteriner, Jurnal Veteriner, Vol. 18, No. 2 ( 2017-06-20), p. 167-
    Abstract: The aim of this study was to evaluate the deoxyribonucleic acid (DNA) status and characteristics of ram spermatozoa, maintained within epididymides stored at 4ºC. A total of 12 pairs of ram cauda epididymis were kept by means that one of the pair in a tube contain media (isotonic NaCl solution) and the other pair into a clean ziplock without medium then stored at 4ºC for three days. Following this, the motility and viability and DNA status of spermatozoa was observed using halomax sperm method, prior and post freezing process. The results showed a decreased in the percentage of motility and viability of spermatozoa from cauda epididymis which were kept in and without media, before or after cryopreservation (P 〈 0.05). Based on the storage method, a significant difference in motility percentage occurs at day two and day three of the storage. Storage using media had a lower percentage of motility (23%; 10%) than without using media (50%; 37%) (P 〈 0.05). The DNA status following storage at 4ºC for three days, descriptively showed impaired of spermatozoa DNA less than 1%. The percentage of impairment increased following the cryopreservation of spermatozoa due to the freezing process. This condition was especially observed on the third day of storage of cauda epididymis which were kept in media (10.16%). The characteristics of spermatozoa from cauda epididymis which were kept in the media were lower compared to without medium. The DNA status of cauda epididymis spermatozoa is not affected by the storage method. ABSTRAK Penelitian ini bertujuan untuk mengevaluasi status DNA dan karakterisik spermatozoa asal kauda epididimis domba yang disimpan pada suhu 4ºC. Sebanyak 12 pasang kauda epididimis domba disimpan dengan cara salah satu dari setiap pasang kauda epididimis dimasukan ke dalam tabung berisi media (NaCl fisiologis) dan pasangan yang lain ke dalam ziplock bersih (tanpa media). Penyimpanan dilakukan selama tiga hari pada suhu 4ºC kemudian dilihat motilitas dan viabilitasnya serta status DNA spermatozoa dengan metode sperm sus halomax, sebelum dan setelah pembekuan. Hasil penelitian menunjukkan bahwa persentase motilitas dan viabilitas spermatozoa asal kauda epididimis baik yang disimpan dengan atau tanpa media mengalami penurunan, sebelum atau setelah kriopreservasi (P 〈 0,05). Berdasarkan metode penyimpanan, perbedaan persentase motilitas yang nyata terjadi pada hari ke dua dan ke tiga penyimpanan. Penyimpanan menggunakan media memiliki persentase motilitas yang lebih rendah (23%; 10%) dibandingkan dengan tanpa menggunakan media (50%; 37%) (P 〈 0,05). Status DNA spermatozoa setelah dilakukan penyimpanan pada suhu 4ºC selama tiga hari, secara deskriptif memerlihatkan kerusakan DNA spermatozoa kurang dari 1%. Persentase kerusakan kemudian meningkat setelah kriopreservasi spermatozoa akibat dari pembekuan, terutama pada hari ke tiga penyimpanan kauda epididimis dengan media (10,16%). Karakteristik spermatozoa dari kauda epididimis yang disimpan dengan media lebih rendah dibandingkan tanpa media. Status DNA spermatozoa kauda epididimis tidak dipengaruhi oleh metode penyimpanan.
    Type of Medium: Online Resource
    ISSN: 2477-5665 , 1411-8327
    URL: Issue
    URL: Article
    DOI: 10.19087/jveteriner.2017.18.2
    DOI: 10.19087/jveteriner.2017.18.2.167
    Language: Unknown
    Publisher: Jurnal Veteriner
    Publication Date: 2017
    detail.hit.zdb_id: 2911720-3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 7
    Online Resource
    Online Resource
    Kafein dalam Medium Maturasi Meningkatkan Fertilisasi dan Menekan Frekuensi Polispermi Oosit Domba dengan Maturasi Diperpanjang (CAFFEINE SUPLEMENTATION IN MATURATION MEDIUM IMPROVE NORMAL FERTILIZATION AND REDUCED THE FREQUENCY OF POLYSPERMY IN SHEEP OOC
    Jurnal Veteriner ; 2017
    In:  Jurnal Veteriner Vol. 18, No. 3 ( 2017-09-04), p. 337-
    Details
    In: Jurnal Veteriner, Jurnal Veteriner, Vol. 18, No. 3 ( 2017-09-04), p. 337-
    Abstract: The objective of this study were to determine kinetic of nuclear maturation and the efficacy of caffeine suplementation in maturation medium on fertilization rate of sheep oocytes in vitro. In the first experiment, oocytes were matured for 16 (M-16), 20 (M-20), 24 (M-24), 28 (M-28) h to assessed the kinetic of oocytes nuclear maturation. In the second experiment, oocytes were matured for 24 h (M-24) or 28 h (M-28) without (M-24 or M-28 groups) or with caffeine suplementation at 4 h before the end of maturation period of oocytes matured for 24 h (M24-Kaf-4) and 28 h (M28-Kaf-4), or 8 h (M28-Kaf-8) before the end of maturation period of oocytes matured for 28 h. Result of the first experiment, 27.6% of oocytes were in metaphase II (MII) at 16 h. The percentage of MII oocytes significantly increased after 20 h (44.8%) to 24 h (88.9%) of maturation period (P 〈 0.05), but the increasing was not found when the maturation period was prolonged until 28 h (89.3%) (P 〉 0.05). However the number of oocytes with two pronucleus (2PN) was higher in group M-24 compared than that of M-28 group (P 〈 0,05) and incidence of polyspermy increased in oocytes of M-28 group (P 〈 0,05). No significant diferences was found in the total of oocytes fertilized among the group except of group M28-Kaf-8 (P 〉 0,05). When caffeine was suplemented at 4 h before the end of maturation period a significantly reduced the incidence of polyspermy and increased the number of oocytes with 2PN in oocytes of M-28 group (P 〈 0.05). In conclusion, the kinetic of nuclear maturation in sheep oocytes showed there was a variation in time required by oocytes to reach MII phase and caffeine improve normal fertilization and reduced the frequency of polyspermy on oocytes when the maturation period prolonged. ABSTRAK Penelitian ini bertujuan untuk mengetahui kinetika maturasi inti dan efektivitas suplementasi kafein pada medium maturasi terhadap tingkat fertilisasi oosit domba secara in vitro. Penelitian tahap I oosit dimaturasi selama 16 (M-16), 20 (M-20), 24 (M-24), dan 28 (M-28) jam untuk mengevaluasi kinetika maturasi inti oosit. Penelitian tahap II, oosit dimaturasi selama 24 jam (M-24) dan 28 jam (M-28) tanpa kafein (kelompok M-24 dan M-28) atau dengan penambahan kafein pada empat jam sebelum akhir periode maturasi pada oosit yang dimaturasi selama 24 jam (M24-Kaf-4) dan 28 jam (M28-Kaf-4), atau delapan jam (M28-Kaf-8) sebelum akhir periode maturasi pada oosit yang dimaturasi selama 28 jam. Hasil penelitian tahap I menunjukkan, 27,6% oosit berada pada tahap metafase II (MII) pada jam ke-16. Persentasi oosit MII meningkat secara signifikan setelah jam ke-20 (44,8%) hingga jam ke-24 (88,9%) periode maturasi (P 〈 0,05) akan tetapi tidak ditemukan adanya peningkatan ketika periode maturasi diperpanjang hingga 28 jam (89,3%) (P 〈 0,05). Namun demikian, jumlah oosit dengan dua pronukleus (2PN) lebih banyak pada kelompok M-24 dibandingkan dengan kelompok M-28 (P 〈 0,05) dan kejadian polispermi meningkat pada oosit kelompok M-28 (P 〈 0,05). Tidak ditemukan adanya perbedaan yang signifikan pada tingkat fertilisasi total antar perlakuan kecuali pada kelompok M28-Kaf-8 (P 〈 0,05). Ketika kafein ditambahkan pada empat jam sebelum akhir periode maturasi secara signifikan dapat menurunkan kejadian polispermi dan meningkatkan jumlah oosit 2PN pada kelompok M-28 (P 〈 0,05). Dapat disimpulkan bahwa kinetika maturasi inti oosit domba menunjukkan ketidakseragaman waktu yang dibutuhkan oleh oosit untuk mencapai tahap MII dan kafein dapat meningkatkan fertilisasi normal dan menurunkan frekuensi polispermi pada oosit ketika periode maturasi diperpanjang
    Type of Medium: Online Resource
    ISSN: 2477-5665 , 1411-8327
    URL: Issue
    URL: Article
    DOI: 10.19087/jveteriner.2017.18.3
    DOI: 10.19087/jveteriner.2017.18.3.337
    Language: Unknown
    Publisher: Jurnal Veteriner
    Publication Date: 2017
    detail.hit.zdb_id: 2911720-3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 8
    Online Resource
    Online Resource
    Daya Fertilisasi Spermatozoa Kauda Epididimis Domba dengan atau tanpa Swim Up sebelum Fertilisasi
    Institut Pertanian Bogor ; 2017
    In:  Acta VETERINARIA Indonesiana Vol. 5, No. 1 ( 2017-07-10), p. 1-7
    Details
    In: Acta VETERINARIA Indonesiana, Institut Pertanian Bogor, Vol. 5, No. 1 ( 2017-07-10), p. 1-7
    Abstract: Penelitian ini dilakukan untuk mengevaluasi penggunaan metode swim up untuk persiapan spermatozoa sebelum fertilisasi terhadap tingkat fertilisasi in vitro spermatozoa kauda epididimis pasca penyimpanan selama 48 jam. Kauda epididimis domba disimpan pada suhu 4 oC selama 0 hari (H-0), 1 hari (H-1) dan 2 hari (H-2), kemudian spermatozoa dikoleksi dan dibekukan. Spermatozoa ejakulat beku digunakan sebagai kontrol. Oosit yang telah matang difertilisasi secara in vitro dengan spermatozoa asal kauda epididimis pasca penyimpanan dan ejakulat menggunakan metode persiapan spermatozoa dengan dan tanpa swim up. Hasil penelitian menunjukkan bahwa spermatozoa asal kauda epididimis yang dikoleksi segera setelah kematian hewan (H-0) memiliki kemampuan yang sama dengan spermatozoa ejakulat (P 〉 0,05). Tingkat fertilisasi spermatozoa kauda epididimis pasca penyimpanan selama 2 hari mengalami penurunan seiring bertambahnya waktu simpan. Penggunaan metode swim up dan tanpa swim up menunjukkan kemampuan fertilisasi yang sama pada spermatozoa ejakulat dan spermatozoa kauda epididimis yang disimpan. Dapat disimpulkan bahwa metode swim up tidak berpengaruh terhadap tingkat fertilisasi in vitro spermatozoa asal kauda epididimis yang disimpan pada suhu 4 °C selama 2 hari. Kemampuan fertilisasi spermatozoa asal kauda epididimis domba yang disimpan pada suhu 4 °C mengalami penurunan sampai hari kedua, namun spermatozoa tersebut masih mampu membuahi oosit secara in vitro.
    Type of Medium: Online Resource
    ISSN: 2337-4373 , 2337-3202
    URL: Article
    DOI: 10.29244/avi.5.1.1-7
    Language: Unknown
    Publisher: Institut Pertanian Bogor
    Publication Date: 2017
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 9
    Online Resource
    Online Resource
    Efektifitas Penambahan Insulin dalam Media Maturasi dan atau Media Kultur pada Tingkat Maturasi Oosit dan Perkembangan Awal Embrio Sapi secara In Vitro
    Universitas Gadjah Mada ; 2019
    In:  Jurnal Sain Veteriner Vol. 37, No. 2 ( 2019-12-11), p. 135-
    Details
    In: Jurnal Sain Veteriner, Universitas Gadjah Mada, Vol. 37, No. 2 ( 2019-12-11), p. 135-
    Abstract: This study was aimed to determine the effect of insulin supplementation in maturation and/or culture medium on nuclear maturation rate and the early bovine embryo development in vitro. Oocytes were collected and matured in maturation media without (IVM I-) or with (IVM I+) 10 ug/µL insulin at incubator 5% CO2 and the temperature of 39 °C, for early embryonic development evaluation, oocytes were divided into 4 groups, without the supplementation of insulin to the maturation medium and culture (IVM I-/IVC I-), insulin supplementation only the maturation medium (IVM I+/IVC I-), insulin supplementation only in the culture medium (IVM I-/IVC I+), and the combination of insulin supplementation to the maturation medium and culture (IVM I+/IVC I+). The result showed that supplementation of insulin to the maturation medium increased number nuclear maturation was higher 87.7% (P 〈 0.05) compared to treatment without supplementation of insulin (70.1%). Cleavage rate in treatment IVM was higher IVM I-/IVC I- (55.8%), IVM I+/IVC I- (64.1%), IVM I-/IVC I+ (59.9%) (P 〈 0.05). Result of the other were showed that early bovine embryo on day-4 cultured (IVC) reached 16 cell on treatment IVM I-/IVC I+(31,9%) and IVM I+/IVC I+ (27,1%) were higher compared to treatment IVM I-/IVC I-(2.9%) and IVM I+/IVC I- (2.5%) (P 〈 0.05). In conclusion, supplementation of insulin to maturation medium and culture medium can increase nuclear maturation rate and improved early embryo cleavage rate.
    Type of Medium: Online Resource
    ISSN: 2407-3733 , 0126-0421
    URL: Article
    DOI: 10.22146/jsv.35565
    Language: Unknown
    Publisher: Universitas Gadjah Mada
    Publication Date: 2019
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 10
    Online Resource
    Online Resource
    Efektivitas Insulin-Like Growth Factor-I (IGF-I) dalam Media Maturasi In Vitro Pada Pematangan Inti dan Fertilisasi Oosit Sapi Bali
    Institut Pertanian Bogor ; 2018
    In:  Acta VETERINARIA Indonesiana Vol. 6, No. 1 ( 2018-10-01), p. 24-29
    Details
    In: Acta VETERINARIA Indonesiana, Institut Pertanian Bogor, Vol. 6, No. 1 ( 2018-10-01), p. 24-29
    Abstract: Penelitian ini bertujuan mengetahui efektivitas insulin-like growth factor–I (IGF-I) pada tingkat pematangan inti dan fertilisasi oosit sapi bali. Penelitian ini dibagi dalam dua tahap. Tahap I, oosit dimatangkan secara in vitro dalam media 199 dengan penambahan 0 (kontrol), 50, 100, dan 150ng/mL IGF-I. Tahap II, oosit dimatangkan dalam media seperti pada penelitian tahap I, kemudian difertilisasi secara in vitro untuk mengamati pembentukan pronukleus. Hasil penelitian tahap I menunjukkan bahwa tidak terdapat perbedaan jumLah oosit yang mencapai tahap metaphase II (MII) dengan penambahan 0 (kontrol), 50, 100, dan 150ng/mL IGF-I dalam media maturasi. Berturut-turut adalah 80,6±7,6%; 81,5±8,6%; 87,5±6,9%; dan 84,1±12,4%. Penelitian tahap II menunjukkan bahwa tingkat fertilisasi pada penambahan 100 ng/mL IGF-I dalam media maturasi nyata lebih tinggi (P 〈 0,05) dibandingkan dengan kontrol dan penambahan 50 ng/mL IGF-I, yaitu berturut-turut 78,3±6,6%, 67,1±8,9%, dan 64,6±6,0% untuk dosis 100, 0, dan 50 ng/mL. Akan tetapi, peningkatan dosis pemberian IGF-I menjadi 150 ng/mL tidak meningkatkan tingkat fertilisasi yaitu 73,5±9,3%. Dari hasil penelitian disimpulkan bahwapenambahan IGF-I dalam media maturasi tidak mampu meningkatkan jumLah oosit yang mencapai tahap MII, namun penambahan 100 ng/mL IGF-I dapat meningkatkan jumLah oosit yang terfertilisasi.
    Type of Medium: Online Resource
    ISSN: 2337-4373 , 2337-3202
    URL: Article
    DOI: 10.29244/avi.6.1.24-29
    Language: Unknown
    Publisher: Institut Pertanian Bogor
    Publication Date: 2018
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...