In:
Disease Markers, Hindawi Limited, Vol. 25, No. 1 ( 2008), p. 27-35
Abstract:
Molecular mechanisms responsible for acute relapse of multiple sclerosis (MS) remain currently unknown. The aim of this study is to identify the relapse-specific biomarker genes in T lymphocytes of relapsing-remitting MS (RRMS). Total RNA of CD3 + T cells isolated from six RRMS patients taken at the peak of acute relapse and at the point of complete remission was processed for DNA microarray analysis. We identified a set of 43 differentially expressed genes (DEG) between acute relapse and complete remission. By using 43 DEG as a discriminator, hierarchical clustering separated the cluster of relapse from that of remission. The molecular network of 43 DEG investigated by KeyMolnet, a bioinformatics tool for analyzing molecular interaction on the curated knowledge database, showed the most significant relationship with aberrant regulation of gene expression by the nuclear factor-kappa B (NF- κ B) in T cells during MS relapse. These results support the logical hypothesis that NF- κ B plays a central role in triggering molecular events in T cells responsible for induction of acute relapse of MS, and suggest that aberrant gene regulation by NF- κ B on T-cell transcriptome might serve as a molecular biomarker for monitoring the clinical disease activity of MS.
Type of Medium:
Online Resource
ISSN:
0278-0240
,
1875-8630
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2008
detail.hit.zdb_id:
2033253-1
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