In:
Future Virology, Future Medicine Ltd, Vol. 12, No. 3 ( 2017-03), p. 93-101
Abstract:
Aim: S16, a monoclonal antibody against the hemagglutinin-esterase fusion (HEF) glycoprotein of influenza C virus, reacts with SV40 large T antigen (LT) and a host cellular component(s). The aim is to determine the location of S16 linear epitope on LT and the amino acid sequence of S16 epitope. Materials & methods: BHK-21 cells expressing wild-type and mutant LTs, HEFs or GFPs, each of which was tagged with a FLAG epitope, were analyzed by immunoblotting using S16. Results & conclusions: An amino acid sequence 98-FNEENL-103 on LT forms a linear epitope recognized by S16. The sequence of S16 epitope was defined as F[NAT]EE[NYA] L, excluding FAEEAL and FTEEAL. This finding will be of help in identifying a host cellular component(s) crossreactive with S16.
Type of Medium:
Online Resource
ISSN:
1746-0794
,
1746-0808
DOI:
10.2217/fvl-2016-0105
Language:
English
Publisher:
Future Medicine Ltd
Publication Date:
2017
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