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  • Wiley  (4)
  • Sangwan, Neelam Singh  (4)
  • 1
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    Online Resource
    Berry transcriptome: insights into a novel resource to understand development dependent secondary metabolism in Withania somnifera (Ashwagandha)
    Wiley ; 2020
    In:  Physiologia Plantarum Vol. 168, No. 1 ( 2020-01), p. 148-173
    Details
    In: Physiologia Plantarum, Wiley, Vol. 168, No. 1 ( 2020-01), p. 148-173
    Abstract: Withania somnifera (Ashwagandha) is considered as Rasayana in Indian systems of medicine. This study reports a novel transcriptome of W. somnifera berries, with high depth, quality and coverage. Assembled and annotated transcripts for nearly all genes related with the withanolide biosynthetic pathway were obtained. Tissue‐wide gene expression analysis reflected almost similar definitions for the terpenoid pathway in leaf, root and berry tissues with relatively higher abundance of transcripts linked to steroid, phenylpropanoid metabolism as well as flavonoid metabolism in berries. The metabolome map generated from the data embodied transcripts from 143 metabolic pathways connected together and mediated collectively by about 1792 unique enzyme functions specific to berry, leaf and root tissues, respectively. Transcripts specific to cytochrome p450 (CYP450), methyltransferases and glycosyltransferases were distinctively located in a tissue specific manner and exhibited a complex network. Significant distribution of transcription factor genes such as MYB, early light inducible protein (ELI), minichromosome maintenance1, agamous, deficiens and serum response factor (MADS) and WRKY etc. was observed, as the major transcriptional regulators of secondary metabolism. Validation of the assembly was ascertained by cloning WsELI , which has a light dependent regulatory role in development. Quantitative expression of WsELI was observed to be considerably modulated upon exposure to abiotic stress and elicitors. Co‐relation of over‐expression of WsELI , may provide new aspects for the functional role of ELI proteins in plants linked to secondary metabolism. The study offers the first comprehensive and comparative evaluation of W. somnifera transcriptome data between the three tissues and across other members of Solanaceae ( Nicotiana , Solanum and Capsicum ) with respect to major pathways and their metabolome regulation.
    Type of Medium: Online Resource
    ISSN: 0031-9317 , 1399-3054
    URL: Issue
    URL: Article
    DOI: 10.1111/ppl.v168.1
    DOI: 10.1111/ppl.12943
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 208872-1
    detail.hit.zdb_id: 2020837-6
    SSG: 12
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  • 2
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    Withanolide A is inherently de novo biosynthesized in roots of the medicinal plant Ashwagandha ( Withania somnifera )
    Wiley ; 2008
    In:  Physiologia Plantarum Vol. 133, No. 2 ( 2008-06), p. 278-287
    Details
    In: Physiologia Plantarum, Wiley, Vol. 133, No. 2 ( 2008-06), p. 278-287
    Abstract: Ashwagandha ( Withania somnifera Dunal., Solanaceae) is one of the most reputed medicinal plants of Ayurveda, the traditional medical system. Several of its traditionally proclaimed medicinal properties have been corroborated by recent molecular pharmacological investigations and have been shown to be associated with its specific secondary metabolites known as withanolides, the novel group of ergostane skeletal phytosteroids named after the plant. Withanolides are structurally distinct from tropane/nortropane alkaloids (usually found in Solanaceae plants) and are produced only by a few genera within Solanaceae. W. somnifera contains many structurally diverse withanolides in its leaves as well as roots. To date, there has been little biosynthetic or metabolism‐related research on withanolides. It is thought that withanolides are synthesized in leaves and transported to roots like the tropane alkaloids, a group of bioactive secondary metabolites in Solanaceae members known to be synthesized in roots and transported to leaves for storage. To examine this, we have studied incorporation of 14 C from [2‐ 14 C]‐acetate and [U‐ 14 C]‐glucose into withanolide A in the in vitro cultured normal roots as well as native/orphan roots of W. somnifera . Analysis of products by thin layer chromatography revealed that these primary metabolites were incorporated into withanolide A, demonstrating that root‐contained withanolide A is de novo synthesized within roots from primary isoprenogenic precursors. Therefore, withanolides are synthesized in different parts of the plant (through operation of the complete metabolic pathway) rather than imported.
    Type of Medium: Online Resource
    ISSN: 0031-9317 , 1399-3054
    URL: Issue
    URL: Article
    DOI: 10.1111/ppl.2008.133.issue-2
    DOI: 10.1111/j.1399-3054.2008.01076.x
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2008
    detail.hit.zdb_id: 208872-1
    detail.hit.zdb_id: 2020837-6
    SSG: 12
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  • 3
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    Carbonate extraction process for the metabolic, isozymic and proteomic profiling of rose‐scented geranium ( Pelargonium sp.), a hyper‐acidic plant
    Wiley ; 2008
    In:  Phytochemical Analysis Vol. 19, No. 2 ( 2008-03), p. 104-115
    Details
    In: Phytochemical Analysis, Wiley, Vol. 19, No. 2 ( 2008-03), p. 104-115
    Abstract: Rose‐scented geranium ( Pelargonium sp.) is a valuable monoterpene‐yielding plant. It has been well characterised phytochemically through the isolation of 〉 270 secondary metabolites, however, there is hardly any biochemical or metabolic information concerning this plant. Initial attempts to investigate its metabolism failed to produce any enzyme activity in the tissue extracts prepared in routine extraction buffers owing to the intrinsic properties of the tissue matrix. It was recognised that cellular hyper‐acidity (cell sap pH ∼3.0) gave rise to very low protein levels in the extracts, thus prohibiting detection of activities of even primary metabolic enzymes that are usually abundantly present in plants. Tissue extraction in Tris solution without pH adjustment (as used for studies involving citrus and banana) led to little or no improvement. Therefore, a novel approach using sodium carbonate solution as an efficient extraction system for enzymes and proteins from the plant was studied. Functionality of the carbonate extraction has been demonstrated through its effectiveness, a several‐fold superior performance, in yielding protein, monitoring primary metabolism and secondary metabolic enzymes, and isozymic and polypeptide profiling. The process may also be helpful in the reliable analysis of other acidic plant tissues. Copyright © 2007 John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 0958-0344 , 1099-1565
    URL: Issue
    URL: Article
    DOI: 10.1002/pca.v19:2
    DOI: 10.1002/pca.1021
    Language: English
    Publisher: Wiley
    Publication Date: 2008
    detail.hit.zdb_id: 1498615-2
    SSG: 12
    Location Call Number Limitation Availability
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  • 4
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    Online Resource
    Analysis of withanolides in root and leaf of Withania somnifera by HPLC with photodiode array and evaporative light scattering detection
    Wiley ; 2008
    In:  Phytochemical Analysis Vol. 19, No. 2 ( 2008-03), p. 148-154
    Details
    In: Phytochemical Analysis, Wiley, Vol. 19, No. 2 ( 2008-03), p. 148-154
    Abstract: A reversed‐phase HPLC method for the simultaneous analysis of nine structurally similar withanolides, namely, 27‐hydroxy withanone, 17‐hydroxy withaferin A, 17‐hydroxy‐27‐deoxy withaferin A, withaferin A, withanolide D, 27‐hydroxy withanolide B, withanolide A, withanone and 27‐deoxywithaferin A, has been developed using a linear binary gradient solvent system comprising methanol and water containing 0.1% acetic acid. Both photodiode array and evaporative light scattering detection were used to profile the extract compositions and to quantify the withanolides therein. Homogeneity and purity of each peak was ascertained by comparative evaluation of the on‐line UV spectra of the eluted compounds with those of the reference compounds. The method has been validated with respect to various parameters of performance quality including computation regression analysis based on calibration curves, peak resolution factor, asymmetry factor, tailing factor, RSD (%) of retention time and peak area response, limit of quantivation, limit of detection, precision and recovery. The developed method has been applied to the analysis of leaf and root tissues of Withania somnifera for withanolide content. Copyright © 2007 John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 0958-0344 , 1099-1565
    URL: Issue
    URL: Article
    DOI: 10.1002/pca.v19:2
    DOI: 10.1002/pca.1029
    Language: English
    Publisher: Wiley
    Publication Date: 2008
    detail.hit.zdb_id: 1498615-2
    SSG: 12
    Location Call Number Limitation Availability
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