In:
RNA, Cold Spring Harbor Laboratory, Vol. 11, No. 10 ( 2005-10), p. 1521-1529
Abstract:
Although it is generally accepted that transcription and translation are spatially separated in eukaryotes, a number of recent observations have called this belief into question. In particular, several studies have shown that parts of the translation machinery, including ribosomal proteins, can be found associated with sites of active transcription in metazoans. Here we describe results of chromatin immunoprecipitation (ChIP) experiments designed to determine whether ribosomal proteins associate with nascent transcripts in Saccharomyces cerevisiae and whether this association reflects a functional engagement of the translation machinery. We find that HAT-tagged ribosomal proteins can be detected in association with nascent RNAs in budding yeast. However, our data clearly indicate that this binding is independent of transcript translatability, so is therefore not indicative of nuclear translation.
Type of Medium:
Online Resource
ISSN:
1355-8382
,
1469-9001
Language:
English
Publisher:
Cold Spring Harbor Laboratory
Publication Date:
2005
detail.hit.zdb_id:
1475737-0
SSG:
12
Permalink
