In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 5 ( 2022-5-25), p. e0263485-
Abstract:
In low-transmission settings, accurate estimates of malaria transmission are needed to inform elimination targets. Detection of antimalarial antibodies provides exposure history, but previous studies have mainly relied on species-specific antigens. The use of chimeric antigens that include epitopes from multiple species of malaria parasites in population-based serological surveys could provide data for exposure to multiple Plasmodium species circulating in an area. Here, the utility of P . vivax/P . falciparum chimeric antigen for assessing serological responses was evaluated in Ethiopia, an endemic country for all four human malarias, and Costa Rica, where P . falciparum has been eliminated with reports of sporadic P . vivax cases. Methods A multiplex bead-based assay was used to determine the seroprevalence of IgG antibodies against a chimeric malaria antigen (PvRMC-MSP1) from blood samples collected from household surveys in Ethiopia in 2015 (n = 7,077) and Costa Rica in 2015 (n = 851). Targets specific for P . falciparum (PfMSP1) and P . vivax (PvMSP1) were also included in the serological panel. Seroprevalence in the population and seroconversion rates were compared among the three IgG targets. Results Seroprevalence in Costa Rica was 3.6% for PfMSP1, 41.5% for PvMSP1 and 46.7% for PvRMC-MSP1. In Ethiopia, seroprevalence was 27.6% for PfMSP1, 21.4% for PvMSP1, and 32.6% for PvRMC-MSP1. IgG levels in seropositive individuals were consistently higher for PvRMC-MSP1 when compared to PvMSP1 in both studies. Seroconversion rates were 0.023 for PvMSP1 and 0.03 for PvRMC-MSP1 in Costa Rica. In Ethiopia, seroconversion rates were 0.050 for PfMSP1, 0.044 for PvMSP1 and 0.106 for PvRMC-MSP1. Conclusions Our data indicate that chimeric antigen PvRMC-MSP1 is able to capture antibodies to multiple epitopes from both prior P . falciparum and P . vivax infections, and suitable chimeric antigens can be considered for use in serosurveys with appropriate validation.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0263485
DOI:
10.1371/journal.pone.0263485.g001
DOI:
10.1371/journal.pone.0263485.g002
DOI:
10.1371/journal.pone.0263485.t001
DOI:
10.1371/journal.pone.0263485.t002
DOI:
10.1371/journal.pone.0263485.s001
DOI:
10.1371/journal.pone.0263485.s002
DOI:
10.1371/journal.pone.0263485.s003
DOI:
10.1371/journal.pone.0263485.s004
DOI:
10.1371/journal.pone.0263485.s005
DOI:
10.1371/journal.pone.0263485.r001
DOI:
10.1371/journal.pone.0263485.r002
DOI:
10.1371/journal.pone.0263485.r003
DOI:
10.1371/journal.pone.0263485.r004
DOI:
10.1371/journal.pone.0263485.r005
DOI:
10.1371/journal.pone.0263485.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2022
detail.hit.zdb_id:
2267670-3
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