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  • Wiley  (4)
  • Reisdorf, Ramona L.  (4)
  • 1
    Online Resource
    Online Resource
    Effects of lubricant and autologous bone marrow stromal cell augmentation on immobilized flexor tendon repairs
    Wiley ; 2016
    In:  Journal of Orthopaedic Research Vol. 34, No. 1 ( 2016-01), p. 154-160
    Details
    In: Journal of Orthopaedic Research, Wiley, Vol. 34, No. 1 ( 2016-01), p. 154-160
    Abstract: The purpose of the study was to test a novel treatment that carbodiimide‐derivatized‐hyaluronic acid‐lubricin (cd‐HA‐lubricin) combined cell‐based therapy in an immobilized flexor tendon repair in a canine model. Seventy‐eight flexor tendons from 39 dogs were transected. One tendon was treated with cd‐HA‐lubricin plus an interpositional graft of 8 × 10 5 BMSCs and GDF‐5. The other tendon was repaired without treatment. After 21 day of immobilization, 19 dogs were sacrificed; the remaining 20 dogs underwent a 21‐day rehabilitation protocol before euthanasia. The work of flexion, tendon gliding resistance, and adhesion score in treated tendons were significantly less than the untreated tendons ( p   〈  0.05). The failure strength of the untreated tendons was higher than the treated tendons at 21 and 42 days ( p   〈  0.05). However, there is no significant difference in stiffness between two groups at day 42. Histologic analysis of treated tendons showed a smooth surface and viable transplanted cells 42 days after the repair, whereas untreated tendons showed severe adhesion formation around the repair site. The combination of lubricant and cell treatment resulted in significantly improved digit function, reduced adhesion formation. This novel treatment can address the unmet needs of patients who are unable to commence an early mobilization protocol after flexor tendon repair. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:154–160, 2016.
    Type of Medium: Online Resource
    ISSN: 0736-0266 , 1554-527X
    URL: Issue
    URL: Article
    DOI: 10.1002/jor.v34.1
    DOI: 10.1002/jor.22980
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2050452-4
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  • 2
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    Revitalized and synovialized allograft for intrasynovial flexor tendon reconstruction in an in vivo canine model
    Wiley ; 2018
    In:  Journal of Orthopaedic Research Vol. 36, No. 8 ( 2018-08), p. 2218-2227
    Details
    In: Journal of Orthopaedic Research, Wiley, Vol. 36, No. 8 ( 2018-08), p. 2218-2227
    Abstract: This study was to test our hypothesis that flexor tendon reconstruction with an allograft revitalized with bone marrow stromal cells (BMSCs) and synovialized with carbodiimide derivatized autologous synovial fluid (cd‐SYN) would result in better digit functional restoration than the conventional allograft tendon. A total of 32 flexor digital profundus tendons from the second and fifth digit of 16 dogs were created a repair failure model first. Then, failed‐repaired tendons were reconstructed with either a revitalized‐synovialized allograft tendon or a clinical standard autograft tendon (control group). The allograft tendon was seeded with autologous BMSCs in multiple slits and the graft surface was coated with cd‐SYN. A 6 weeks after tendon reconstruction, the digits were harvested and evaluated for digit function, adhesion status, tendon gliding resistance, attachment strength, cell viability, and histologic factors. The allograft group had significantly improved digit function compared with the control group through decreased work of flexion, increased digit range of motion under 2‐Newton force, and less adhesion score ( p   〈  .05). However, the distal attachment‐site strength and stiffness in the allograft tendon were significantly weaker than the autografts ( p   〈  .05). No significant difference was found for gliding resistance. Histologically, allograft tendons coated with allograft had smoother surfaces and showed tendon‐to‐bone and tendon‐to‐tendon incorporation. Viable BMSCs were found in the tendon slits 6 weeks after the graft. In conclusion, cellular lubricant‐based modification of allograft tendons improved digit function and reduced the adhesions compared with autograft for flexor tendon reconstruction. However, improvement of graft‐to‐host tendon healing is still challenging. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:2218–2227, 2018.
    Type of Medium: Online Resource
    ISSN: 0736-0266 , 1554-527X
    URL: Issue
    URL: Article
    DOI: 10.1002/jorr.v36.8
    DOI: 10.1002/jor.23889
    Language: English
    Publisher: Wiley
    Publication Date: 2018
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  • 3
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    Biomechanical evaluation of flexor tendon graft with different repair techniques and graft surface modification
    Wiley ; 2015
    In:  Journal of Orthopaedic Research Vol. 33, No. 5 ( 2015-05), p. 731-737
    Details
    In: Journal of Orthopaedic Research, Wiley, Vol. 33, No. 5 ( 2015-05), p. 731-737
    Abstract: The purpose of this study was to investigate the biomechanical properties of modified repair techniques for flexor tendon reconstruction and the effects of surface modification using carbodiimide‐derivatized synovial fluid plus gelatin (cd‐SF‐G), compared to the traditional repair techniques. The second and fifth digits from 16 canine forepaws were randomly divided into 4 groups: (1) traditional graft repairs (TGR group) including distal Bunnell repair and proximal Pulvertaft weave repair; (2) modified graft repairs (MGR group) including distal graft bony attachment repair and proximal step‐cut repair; (3) group TGR coated with cd‐SF‐G (TGR‐C group); and (4) group MGR coated with cd‐SF‐G (MGR‐C group). Digit normalized work of flexion (nWOF), ultimate failure strength, and stiffness were measured. The nWOF in MGR group was significantly less than TGR group ( p   〈  0.05). The nWOF in groups treated with cd‐SF‐G was significantly less than their untreated counterparts (p   〈  0.05). Ultimate load to failure of the MGR‐C group was significantly greater than the TGR‐C group ( p   〈  0.05), but no significant difference in stiffness was found between these two groups. The modified techniques cannot only improve tendon gliding abilities but can also improve breaking strength. Additionally, surface modification with cd‐SF‐G significantly decreased the work of flexion. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:731–737, 2015.
    Type of Medium: Online Resource
    ISSN: 0736-0266 , 1554-527X
    URL: Issue
    URL: Article
    DOI: 10.1002/jor.v33.5
    DOI: 10.1002/jor.22844
    Language: English
    Publisher: Wiley
    Publication Date: 2015
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  • 4
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    Characterization of a purified exosome product and its effects on canine flexor tenocyte biology
    Wiley ; 2020
    In:  Journal of Orthopaedic Research Vol. 38, No. 8 ( 2020-08), p. 1845-1855
    Details
    In: Journal of Orthopaedic Research, Wiley, Vol. 38, No. 8 ( 2020-08), p. 1845-1855
    Abstract: Flexor tendon injuries and tendinopathy are very common but remain challenging in clinical treatment. Exosomes‐based cell‐free therapy appears to be a promising strategy for tendon healing, while limited studies have evaluated its impacts on tenocyte biology. The objective of this study was to characterize a novel purified exosome product (PEP) derived from plasma, as well as to explore its cellular effects on canine tenocyte biology. The transmission electron microscope revealed that exosomes of PEP present cup‐shaped structures with the diameters ranged from 80 to 141 nm, and the NanoSight report presented that their size mainly concentrated around 100 nm. The enzyme‐linked immunosorbent assay kits analysis showed that PEP was positive for CD63 and AChE expression, and the cellular uptake of exosomes internalized into tenocyte cytoplasm was observed. The cell growth assays displayed that tenocyte proliferation ability was enhanced by PEP solution in a dose‐dependent manner. Tenogenic phenotype was preserved as is evident by that tendon‐related genes expression (SCX, COL1A, COL3A1, TNMD, DCN, and MKX) were expressed insistently in a high level, while tenocytes were treated with 5% PEP solution. Furthermore, migration capability was maintained and total collagen deposition was increased. More interesting, dexamethasone‐induced cellular apoptosis was attenuated during the incubation of tenocytes with a 5% PEP solution. These findings will provide the basic understandings about the PEP, and support the potential use of this biological strategy for tendon healing.
    Type of Medium: Online Resource
    ISSN: 0736-0266 , 1554-527X
    URL: Issue
    URL: Article
    DOI: 10.1002/jor.v38.8
    DOI: 10.1002/jor.24587
    Language: English
    Publisher: Wiley
    Publication Date: 2020
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