In:
OBM Genetics, LIDSEN Publishing Inc, Vol. 07, No. 03 ( 2023-07-20), p. 1-14
Abstract:
〈 em 〉 Hedysarum cretaceum 〈 /em 〉 Fisch 〈 em 〉 〈 /em 〉 is a critically endangered herbaceous species with high ornamental and phytomeliorative properties; thus, it needs urgent conservation and protection methods to conserve. In the present study, an efficient method for microtonal propagation of 〈 em 〉 H. cretaceum 〈 /em 〉 is developed. The seed sterilization was optimized as treatment with 96% alcohol (2 min) and burned (1 sec) through a burner flame. Murashige and Skoog (MS) medium with the addition of 0.5 mg/L 6-Benzylaminopurine (BAP) (shoots per explant – 18.0) and 1.0 mg/L BAP and 0.1 mg/L Indole-3-acetic acid (IAA) (shoots per explant – 18.4) for the shoot and ⅓ MS supplemented with 0.1 mg/L IAA (rooting indicator – 62%) for roots were identified best composition for the proliferation and regeneration. Additionally, the medium for maintaining callus culture was determined – DKW with the addition of 2.5 mg/L BAP and 0.1 mg/L IAA, the possibility of direct organogenesis 〈 em 〉 in vitro 〈 /em 〉 in the studied plant was also standardized. In addition, seed germination after various pre-sowing treatments was studied. It is shown that scarification makes achieving 80% germination of seeds possible. Also, sulfuric acid was used as a pre-sowing seed treatment, germination was 45%. Thus, using the protocols we developed, it is possible to maintain the 〈 em 〉 H. cretaceum 〈 /em 〉 culture 〈 em 〉 in vitro 〈 /em 〉 and increase seed germination using pre-sowing treatment.
Type of Medium:
Online Resource
ISSN:
2577-5790
DOI:
10.21926/obm.genet.2303
DOI:
10.21926/obm.genet.2303187
Language:
Unknown
Publisher:
LIDSEN Publishing Inc
Publication Date:
2023
detail.hit.zdb_id:
3009912-2
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