In:
Journal of Oncology, Hindawi Limited, Vol. 2020 ( 2020-08-25), p. 1-8
Abstract:
Background . Inhibitors of apoptosis proteins such as cIAP-1 and cIAP-2 have recently emerged as the key mechanism in resistance to apoptosis in various cancers and lead to cell survival. Therefore, the present study aimed to evaluate the cIAP-1 and cIAP-2 expression in breast cancer patients, as well as their association with overall patient survival. Methods . Histopathologically confirmed 100 invasive ductal carcinoma patients and healthy controls were included in the present study. Total RNA extraction was done from the serum sample of the patients; further, 100 ng of total RNA was used to synthesise cDNA from patients’ as well as from healthy controls’ serum. Quantitative real-time PCR was performed using the maxima SYBR Green dye to study the expression of cIAP-1 and cIAP-2, and beta-actin was used as the internal control. Results . The study observed that breast cancer patients had 13.50 mean fold increased cIAP-1 mRNA and 8.76 mean fold increased cIAP-2 mRNA expression compared to the control subjects. Breast cancer patients in the TNM stages I, II, III, and IV showed 9.54, 11.80, 15.19, and 16.83 mean fold increased cIAP-1 mRNA expression ( p = 0.004 ). Distant organ metastasis, ( p = 0.008 ), PR status of breast cancer patients ( p 〈 0.0001 ), and HER2 status of breast cancer patients ( p 〈 0.0001 ) were found to be associated with cIAP-1 mRNA expression. Breast cancer patients with different TNM stages such as stages I, II, III, and IV showed 7.8, 8.09, 7.97, and 12.85 mean fold increased cIAP-2 mRNA expression ( p = 0.0002 ). Breast cancer patients with distant organ metastases status were found to be associated with cIAP-2 mRNA expression ( p 〈 0.0001 ). Breast cancer patients with 〈 13-fold and 〉 13-fold cIAP-1 mRNA expression showed 37.39 months and 34.70 months of overall median survival, and the difference among them was found to be significant ( p = 0.0001 ). However, cIAP-2 mRNA expression among 〈 8-fold and 〉 8-fold mRNA expression groups showed 35 months and 27.90 months of overall median survival time ( p 〈 0.0001 ). Higher cIAP-1 mRNA expression was linked with smoking and alcoholism among the breast cancer patients ( p 〈 0.0001 and p 〈 0.0001 ). Significant association of higher cIAP-1 mRNA expression was found with the advancement of the disease, while higher mRNA expression of cIAP-1 was associated with distant organ metastases in ROC curve analysis. Conclusion . The present study suggested that increased cell-free cIAP-1 and cIAP-2 mRNA expression was correlated with the advancement of disease, progression of disease, and overall reduced patient survival. Cell-free cIAP-1 and cIAP-2 mRNA expression could be the predictive indicator of the disease.
Type of Medium:
Online Resource
ISSN:
1687-8450
,
1687-8469
DOI:
10.1155/2020/3634825
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2020
detail.hit.zdb_id:
2461349-6
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