In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 7 ( 2021-7-8), p. e0253975-
Abstract:
Diagnostic ratios calculated from urinary steroid hormone metabolites are used as a measure for the relative activity of steroidogenic enzymes or pathways in the clinical investigation of steroid metabolism disorders. However, population-based sex- and age-specific reference intervals and day-night differences in adults are lacking. Methods Sixty-five diagnostic ratios were calculated from steroid metabolites measured by GC-MS in day- and night-time and in 24-hour urine from 1128 adults recruited within the Swiss Kidney Project on Genes in Hypertension (SKIPOGH), a population-based, multicenter cohort study. Differences related to sex, age and day- and night-time were evaluated and reference curves in function of age and sex were modelled by multivariable linear mixed regression for diagnostic ratios and were compared to values from the literature. Results Most ratios had sex- and age-specific relationships. For each ratio, percentiles were plotted in function of age and sex in order to create reference curves and sex- and age-specific reference intervals derived from 2.5 th and 97.5 th percentiles were obtained. Most ratios reflected a higher enzyme activity during the day compared to the night. Conclusions Sex- and age-specific references for 24 hours, day and night urine steroid metabolite ratios may help distinguishing between health and disease when investigating human disorders affecting steroid synthesis and metabolism. The day-night differences observed for most of the diagnostic ratios suggest a circadian rhythm for enzymes involved in human steroid hormones metabolism.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0253975
DOI:
10.1371/journal.pone.0253975.g001
DOI:
10.1371/journal.pone.0253975.t001
DOI:
10.1371/journal.pone.0253975.t002
DOI:
10.1371/journal.pone.0253975.t003
DOI:
10.1371/journal.pone.0253975.s001
DOI:
10.1371/journal.pone.0253975.s002
DOI:
10.1371/journal.pone.0253975.s003
DOI:
10.1371/journal.pone.0253975.s004
DOI:
10.1371/journal.pone.0253975.s005
DOI:
10.1371/journal.pone.0253975.s006
DOI:
10.1371/journal.pone.0253975.s007
DOI:
10.1371/journal.pone.0253975.s008
DOI:
10.1371/journal.pone.0253975.s009
DOI:
10.1371/journal.pone.0253975.s010
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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