In:
Journal of Cellular and Molecular Medicine, Wiley, Vol. 21, No. 10 ( 2017-10), p. 2403-2411
Abstract:
Septic shock is a common medical condition with a mortality approaching 50% where early diagnosis and treatment are of particular importance for patient survival. Novel biomarkers that serve as prompt indicators of sepsis are urgently needed. High‐throughput technologies assessing circulating micro RNA s represent an important tool for biomarker identification, but the blood‐compartment specificity of these mi RNA s has not yet been investigated. We characterized mi RNA profiles from serum exosomes, total serum and blood cells (leukocytes, erythrocytes, platelets) of sepsis patients by next‐generation sequencing and RT ‐ qPCR ( n = 3 × 22) and established differences in mi RNA expression between blood compartments. In silico analysis was used to identify compartment‐specific signalling functions of differentially regulated mi RNA s in sepsis‐relevant pathways. In septic shock, a total of 77 and 103 mi RNA s were down‐ and up‐regulated, respectively. A majority of these regulated mi RNA s (14 in serum, 32 in exosomes and 73 in blood cells) had not been previously associated with sepsis. We found a distinctly compartment‐specific regulation of mi RNA s between sepsis patients and healthy volunteers. Blood cellular miR‐199b‐5p was identified as a potential early indicator for sepsis and septic shock. miR‐125b‐5p and miR‐26b‐5p were uniquely regulated in exosomes and serum, respectively, while one mi RNA (miR‐27b‐3p) was present in all three compartments. The expression of sepsis‐associated mi RNA s is compartment‐specific. Exosome‐derived mi RNA s contribute significant information regarding sepsis diagnosis and survival prediction and could serve as newly identified targets for the development of novel sepsis biomarkers.
Type of Medium:
Online Resource
ISSN:
1582-1838
,
1582-4934
DOI:
10.1111/jcmm.2017.21.issue-10
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2076114-4
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