In:
Journal of Applied Crystallography, International Union of Crystallography (IUCr), Vol. 53, No. 2 ( 2020-04-01), p. 477-485
Abstract:
Microcrystal delivery methods are pivotal in the use of serial femtosecond crystallography (SFX) to resolve the macromolecular structures of proteins. Here, the development of a novel technique and instruments for efficiently delivering microcrystals for SFX are presented. The new method, which relies on a one-dimensional fixed-target system that includes a microcrystal container, consumes an extremely low amount of sample compared with conventional two-dimensional fixed-target techniques at ambient temperature. This novel system can deliver soluble microcrystals without highly viscous carrier media and, moreover, can be used as a microcrystal growth device for SFX. Diffraction data collection utilizing this advanced technique along with a real-time visual servo scan system has been successfully demonstrated for the structure determination of proteinase K microcrystals at 1.85 Å resolution.
Type of Medium:
Online Resource
ISSN:
1600-5767
DOI:
10.1107/S1600576720002423
DOI:
10.1107/S1600576720002423/yr5056sup1.mp4
DOI:
10.1107/S1600576720002423/yr5056sup2.pdf
Language:
Unknown
Publisher:
International Union of Crystallography (IUCr)
Publication Date:
2020
detail.hit.zdb_id:
2020879-0
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