In:
PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 2 ( 2022-2-24), p. e0263310-
Kurzfassung:
Broccoli ( Brassica oleracea var. italica ) is an important B . oleracea cultivar, with high economic and agronomic value. However, comparative genome analyses are still needed to clarify variation among cultivars and phylogenetic relationships within the family Brassicaceae. Herein, the complete chloroplast (cp) genome of broccoli was generated by Illumina sequencing platform to provide basic information for genetic studies and to establish phylogenetic relationships within Brassicaceae. The whole genome was 153,364 bp, including two inverted repeat (IR) regions of 26,197 bp each, separated by a small single copy (SSC) region of 17,834 bp and a large single copy (LSC) region of 83,136 bp. The total GC content of the entire chloroplast genome accounts for 36%, while the GC content in each region of SSC,LSC, and IR accounts for 29.1%, 34.15% and 42.35%, respectively. The genome harbored 133 genes, including 88 protein-coding genes, 37 tRNAs, and 8 rRNAs, with 17 duplicates in IRs. The most abundant amino acid was leucine and the least abundant was cysteine. Codon usage analyses revealed a bias for A/T-ending codons. A total of 35 repeat sequences and 92 simple sequence repeats were detected, and the SC-IR boundary regions were variable between the seven cp genomes. A phylogenetic analysis suggested that broccoli is closely related to Brassica oleracea var. italica MH388764.1, Brassica oleracea var. italica MH388765.1, and Brassica oleracea NC_0441167.1. Our results are expected to be useful for further species identification, population genetics analyses, and biological research on broccoli.
Materialart:
Online-Ressource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0263310
DOI:
10.1371/journal.pone.0263310.g001
DOI:
10.1371/journal.pone.0263310.g002
DOI:
10.1371/journal.pone.0263310.g003
DOI:
10.1371/journal.pone.0263310.g004
DOI:
10.1371/journal.pone.0263310.g005
DOI:
10.1371/journal.pone.0263310.t001
DOI:
10.1371/journal.pone.0263310.t002
DOI:
10.1371/journal.pone.0263310.t003
DOI:
10.1371/journal.pone.0263310.t004
DOI:
10.1371/journal.pone.0263310.t005
DOI:
10.1371/journal.pone.0263310.t006
DOI:
10.1371/journal.pone.0263310.t007
DOI:
10.1371/journal.pone.0263310.t008
DOI:
10.1371/journal.pone.0263310.t009
Sprache:
Englisch
Verlag:
Public Library of Science (PLoS)
Publikationsdatum:
2022
ZDB Id:
2267670-3
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