In:
Pest Management Science, Wiley, Vol. 78, No. 9 ( 2022-09), p. 3871-3879
Kurzfassung:
Use of RNA interference (RNAi) technology in effective pest management has been explored for decades. Henosepilachna vigintioctopunctata is a major solanaceous crop pest in Asia. In this study, the effects of the RNAi‐mediated silencing of clathrin heavy chain in H. vigintioctopunctata were investigated. RESULTS Feeding either the in vitro ‐synthesized or the bacterially expressed double‐stranded RNAs (dsRNAs) significantly impaired the normal physiology of H. vigintioctopunctata instars and adults. However, the bacterially expressed ds HvChc caused higher mortality than the in vitro ‐synthesized ones in the larvae and adults. Moreover, on evaluating the potential risk of ds HvChc on Propylea japonica , significant transcriptional effects of ds HvChc1 were observed, while the organismal level effects were not significant. On the contrary, ds HvChc2 did not affect P. japonica at either level. A similar test revealed significant transcriptional effects of ds PjChc1 on H. vigintioctopunctata , while staying ineffective at the organismal levels. Conversely, ds PjChc2 did not affect H. vigintioctopunctata at either level. Importantly, no effect of ds PjChc1 exposure on H. vigintioctopunctata suggested that other factors besides the 21‐nucleotide (nt) matches between sequences were responsible. Finally, ingestion of ds HvmChc1 derived from H. vigintioctomaculata , containing 265‐nt matches with ds HvChc1 , caused 100% mortality in H. vigintioctopunctata . CONCLUSIONS We conclude that (i) species with numerous 21‐nt matches in homologous genes are more likely to be susceptible to dsRNA; (ii) dsRNA can be safely designed to avoid negative effects on non‐target organisms at both transcriptional and organismal levels; (iii) HvChc can be used as an efficient RNAi target gene to effectively manage H. vigintioctopunctata . © 2021 Society of Chemical Industry.
Materialart:
Online-Ressource
ISSN:
1526-498X
,
1526-4998
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2022
ZDB Id:
2003455-6
SSG:
12
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