In:
PLOS Neglected Tropical Diseases, Public Library of Science (PLoS), Vol. 14, No. 12 ( 2020-12-7), p. e0008952-
Abstract:
The liver fluke Clonorchis sinensis inhabits the bile ducts, where bile concentration disparities across the fluke cell membrane can cause bile intoxication. Sodium-bile acid co-transporter (SBAT) plays a crucial role in bile acid recycling. The process by which SBAT imports bile acids is electrically coupled to sodium ion co-transportation. Here, we report that the SBAT of C . sinensis (CsSBAT) is involved in bile acid transportation. CsSBAT cDNA encoded a putative polypeptide of 546 amino acid residues. Furthermore, CsSBAT consisted of ten putative transmembrane domains, and its 3D structure was predicted to form panel and core domains. The CsSBAT had one bile acid- and three Na + -binding sites, enabling coordination of a symport process. CsSBAT was mainly localized in the mesenchymal tissue throughout the fluke body and sparsely localized in the basement of the tegument, intestinal epithelium, and excretory bladder wall. Bile acid permeated into the adult flukes in a short time and remained at a low concentration level. Bile acid accumulated inside the mesenchymal tissue when CsSBAT was inhibited using polyacrylic acid–tetradeoxycholic acid conjugate. The accumulated bile acid deteriorated the C . sinensis adults leading to death. CsSBAT silencing shortened the lifespan of the fluke when it was placed into bile. Taken together, we propose that CsSBAT transports bile acids in the mesenchymal tissue and coordinate with outward transporters to maintain bile acid homeostasis of C . sinensis adults, contributing to C . sinensis survival in the bile environment.
Type of Medium:
Online Resource
ISSN:
1935-2735
DOI:
10.1371/journal.pntd.0008952
DOI:
10.1371/journal.pntd.0008952.g001
DOI:
10.1371/journal.pntd.0008952.g002
DOI:
10.1371/journal.pntd.0008952.g003
DOI:
10.1371/journal.pntd.0008952.g004
DOI:
10.1371/journal.pntd.0008952.g005
DOI:
10.1371/journal.pntd.0008952.g006
DOI:
10.1371/journal.pntd.0008952.g007
DOI:
10.1371/journal.pntd.0008952.g008
DOI:
10.1371/journal.pntd.0008952.g009
DOI:
10.1371/journal.pntd.0008952.s001
DOI:
10.1371/journal.pntd.0008952.s002
DOI:
10.1371/journal.pntd.0008952.s003
DOI:
10.1371/journal.pntd.0008952.s004
DOI:
10.1371/journal.pntd.0008952.s005
DOI:
10.1371/journal.pntd.0008952.s006
DOI:
10.1371/journal.pntd.0008952.s007
DOI:
10.1371/journal.pntd.0008952.s008
DOI:
10.1371/journal.pntd.0008952.s009
DOI:
10.1371/journal.pntd.0008952.s010
DOI:
10.1371/journal.pntd.0008952.s011
DOI:
10.1371/journal.pntd.0008952.s012
DOI:
10.1371/journal.pntd.0008952.s013
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2020
detail.hit.zdb_id:
2429704-5
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