In:
European Journal of Pain, Wiley, Vol. 16, No. 10 ( 2012-11), p. 1368-1377
Abstract:
Chronic pain of neuropathic nature after spinal cord injury ( SCI ) is common and its underlying mechanisms are poorly understood. Genes, as well as sex, have been implicated, but not thoroughly investigated in experimental genetic models for complex traits. We have previously found that inbred D ark‐ A gouti ( DA ) rats develop more severe SCI pain‐like behaviour than a major histocompatibility complex‐congenic Piebald Virol Glaxo ( PVG )‐ RT 1 av1 strain in a model of photochemically induced SCI . Methods In this study, a genome‐wide linkage study in an F 2 cross between the susceptible DA and resistant PVG ‐ RT 1 av1 strains was performed in order to explore the influence of genes and sex for SCI pain. Results A consistent finding was that female rats in parental, F 1 and F 2 generations displayed increased pain sensitivity at testing before injury and also developed mechanical hypersensitivity more rapidly and to a greater extent than male rats. In addition, we could identify three quantitative trait loci ( QTLs ) associated with pain‐like behaviour: a sex‐specific QTL on chromosome 2, one on chromosome 15 and on chromosome 6. Animals carrying DA alleles at each of these loci were more susceptible to development of mechanical hypersensitivity compared with rats with PVG alleles. Conclusion This is the first whole genome QTL mapping of neuropathic pain‐like behaviour in a model of SCI . The results provide strong support for a significant genetic and sex component in development of pain after SCI and provide the basis for further genetic dissection and positional cloning of the underlying genes.
Type of Medium:
Online Resource
ISSN:
1090-3801
,
1532-2149
DOI:
10.1002/ejp.2012.16.issue-10
DOI:
10.1002/j.1532-2149.2012.00144.x
Language:
English
Publisher:
Wiley
Publication Date:
2012
detail.hit.zdb_id:
2002493-9
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