In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 64, No. Pt_8 ( 2014-08-01), p. 2605-2610
Abstract:
Two actinomycete strains, KK1-2 T and CPB4-7, were isolated from marine sediments collected in Chumphon province, Thailand. Chumphon province, Thailand. Their taxonomic positions were determined using a polyphasic approach. The morphological, cultural and chemotaxonomic characteristics of these isolates were consistent with the classification of the strains as representing a member of the genus Streptomyces . They contained ll -diaminopimelic acid in their cell wall peptidoglycan; the whole-cell sugars were ribose and glucose. The predominant menaquinones were MK9-(H 6 ) and MK9-(H 8 ). The major polar lipids were phosphatidylethanolamine, phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides. The predominant cellular fatty acids were anteiso-C 15 : 0 , iso-C 16 : 0 and iso-C 15 : 0 . On the basis of 16S rRNA gene sequence similarity studies, these isolates were determined to be closely related to Streptomyces xinghaiensis JCM 16958 T (98.2 %), Streptomyces rimosus subsp. paromomycinus JCM 4541 T (98.1 %), Streptomyces sclerotialus JCM 4828 T (98.1 %) and Streptomyces flocculus JCM 4476 T (98.0 %). The G+C contents of the genomic DNA of strains KK1-2 T and CPB4-7 were 73.3 and 74.2 mol%, respectively. They could be clearly distinguished from the related type strains by a low DNA–DNA relatedness and phenotypic differences. On the basis of these results, these strains represent a novel species of the genus Streptomyces , for which the name Streptomyces chumphonensis sp. nov. (type strain KK1-2 T = JCM 18522 T = TISTR 2106 T = PCU 330 T ) is proposed.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.062992-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2014
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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