In:
PLOS ONE, Public Library of Science (PLoS), Vol. 15, No. 12 ( 2020-12-2), p. e0242880-
Abstract:
Udder cleft dermatitis (UCD) is a skin condition affecting the fore udder attachment of dairy cows. UCD may be defined as mild (eczematous skin changes) or severe (open wounds, large skin changes). Our aims were to compare the microbiota of mild and severe UCD lesions with the microbiota of healthy skin from the fore udder attachment of control cows, and to investigate whether mastitis-causing pathogens are present in UCD lesions. Samples were obtained from cows in six dairy herds. In total, 36 UCD samples categorized as mild (n = 17) or severe (n = 19) and 13 control samples were sequenced using a shotgun metagenomic approach and the reads were taxonomically classified based on their k -mer content. The Wilcoxon rank sum test was used to compare the abundance of different taxa between different sample types, as well as to compare the bacterial diversity between samples. A high proportion of bacteria was seen in all samples. Control samples had a higher proportion of archaeal reads, whereas most samples had low proportions of fungi, protozoa and viruses. The bacterial microbiota differed between controls and mild and severe UCD samples in both composition and diversity. Subgroups of UCD samples were visible, characterized by increased proportion of one or a few bacterial genera or species, e.g. Corynebacterium , Staphylococcus , Brevibacterium luteolum , Trueperella pyogenes and Fusobacterium necrophorum . Bifidobacterium spp. were more common in controls compared to UCD samples. The bacterial diversity was higher in controls compared to UCD samples. Bacteria commonly associated with mastitis were uncommon. In conclusion, a dysbiosis of the microbiota of mild and severe UCD samples was seen, characterized by decreased diversity and an increased proportion of certain bacteria. There was no evidence of a specific pathogen causing UCD or that UCD lesions are important reservoirs for mastitis-causing bacteria.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0242880
DOI:
10.1371/journal.pone.0242880.g001
DOI:
10.1371/journal.pone.0242880.g002
DOI:
10.1371/journal.pone.0242880.g003
DOI:
10.1371/journal.pone.0242880.g004
DOI:
10.1371/journal.pone.0242880.g005
DOI:
10.1371/journal.pone.0242880.g006
DOI:
10.1371/journal.pone.0242880.g007
DOI:
10.1371/journal.pone.0242880.g008
DOI:
10.1371/journal.pone.0242880.t001
DOI:
10.1371/journal.pone.0242880.s001
DOI:
10.1371/journal.pone.0242880.s002
DOI:
10.1371/journal.pone.0242880.s003
DOI:
10.1371/journal.pone.0242880.s004
DOI:
10.1371/journal.pone.0242880.s005
DOI:
10.1371/journal.pone.0242880.s006
DOI:
10.1371/journal.pone.0242880.s007
DOI:
10.1371/journal.pone.0242880.r001
DOI:
10.1371/journal.pone.0242880.r002
DOI:
10.1371/journal.pone.0242880.r003
DOI:
10.1371/journal.pone.0242880.r004
DOI:
10.1371/journal.pone.0242880.r005
DOI:
10.1371/journal.pone.0242880.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2020
detail.hit.zdb_id:
2267670-3
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