In:
Plant Breeding, Wiley, Vol. 132, No. 6 ( 2013-12), p. 701-710
Abstract:
Plant 14‐3‐3 proteins are involved in signal transduction pathways of nitrogen and carbohydrate metabolism. An Eg14‐3‐3 ω gene was isolated from the mesocarp of oil palm. The 1055‐bp c DNA had an open reading frame of 774 bp that encoded for 258 amino acids, and the c DNA had 113‐bp and 195‐bp 5′‐ and 3′‐untranslated regions, respectively. The calculated molecular weight was 28.06 kDa, with a pI of 5.04. The palm 14‐3‐3 showed closest identity to 14‐3‐3 proteins of the omega group. The entire sequence of Eg14‐3‐3 ω showed 83% identity with 14‐3‐3 protein isoform 16R from S olanum tuberosum . Phylogenetic analysis showed that the Eg14‐3‐3 isoform was within the omega ( ω ) subgroup and, thus, was designated Eg14‐3‐3 ω . The Eg14‐3‐3 ω expression patterns were strong in the mesocarp as compared to the root. When Eg14‐3‐3 ω c DNA was overexpressed in transgenic calli, there was higher accumulation of oil in the transgenic calli than in the controls. Therefore, Eg14‐3‐3 ω has potential for applications in the breeding of oil palms in the future.
Type of Medium:
Online Resource
ISSN:
0179-9541
,
1439-0523
DOI:
10.1111/pbr.2013.132.issue-6
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
2020488-7
SSG:
12
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