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  • Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)  (6)
  • Nhut, Duong Tan  (6)
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  • Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)  (6)
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  • 1
    Online Resource
    Online Resource
    Improved in vitro rooting and acclimatization of “Violetta” Artichoke and “Green Globe” Artichoke
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2021
    In:  Vietnam Journal of Biotechnology Vol. 19, No. 1 ( 2021-07-18), p. 129-145
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 19, No. 1 ( 2021-07-18), p. 129-145
    Abstract: Artichoke (Cynara scolymus L.), a medicinal plant with high economic value, contains high levels of phenolic compounds; especially cynarine, which plays an important role in preventing cancer, cardiovascular disease, osteoporosis, diabetes and neurodegeneration, etc. Currently, Artichoke micropropagation has achieved some success; however, the rooting efficiency and plantlet quality are still limited. In this study, improving the quality of Artichoke plantlet related to the shoot quality and suitable substrates in in vitro rooting stage was studied on “Violetta” Artichoke (VA) and “Green Globe” Artichoke (GA). The results showed that shoots (1.5 cm) cultured on MS medium supplemented 0.5 mg/L KIN were most suitable to shoot multiplication of VA with the number of shoots/explant (3.67 shoots), number of shoots ≥ 2 cm (3 shoots); while, 1.0 mg/L BA was suitable to shoot multiplication of GA (5.33 shoots; 5.00 shoots; respectively) after 4 weeks of culture. Besides, the in vitro rooting was improved using 8 g/L commercial agar for VA; meanwwhile, 3 g/L gelrite for GA. In addition, the nylon bag culture system (120 mm × 250 mm) has potential in plantlet production (15 plants/bag) and can be applied for large scale micropropagation. In addition, VA and GA plantlets derived from in vitro culture gave the good acclimatization, growth and development after 8, 12 and 20 weeks cultivating at the green house conditions.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/15204
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2021
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    Online Resource
  • 2
    Online Resource
    Online Resource
    Effect of silver nanoparticles on sterilization of different explant sources of Gerbera jamesonii cultured in vitro
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2022
    In:  Vietnam Journal of Biotechnology Vol. 19, No. 4 ( 2022-05-03), p. 705-715
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 19, No. 4 ( 2022-05-03), p. 705-715
    Abstract: In this study, silver nanoparticles effects on the sterilization of different sources of explants (young leaves, young flower stalks and young flower buds) of Gerbera as well as on the in vitro morphogenesis and their growth were investigated. The explants were sterilized and cut transversally (1 mm) with the flower stalk, square (0.5 × 0.5 cm) for the leaf sample, longitudinally (0.5 mm) for the flowers (removed the petals) and cultured on MS medium; then, the explants (contamination-free or no browning/necrosis) were transferred into MS medium supplemented with 0.02 mg/L TDZ plus 0.8 mg/L adenine, 10% coconut water, 30 g/L sucrose and 8 g/L agar in 15 days. The results showed that AgNPs at the appropriate concentration and duration treatment was effective in explant sterilization of flower bud (0.02% AgNPs and 20 min), flower stalks (0.02% AgNPs and 30 min) and young leaves (0.05% AgNPs and 20 min) after 15 days of culture. In addition, 3 types of morphogenesis including callus induction, somatic embryogenesis and direct shoot regeneration of explants derived from sterilization by AgNPs were improved as compared to that of HgCl2. In addition, research on the optimal medium for shoot multiplication, rooting as well as evaluation of acclimatization and the growth at the greenhouse were also studied. Results showed that MS medium supplemented with 2 mg/L NAA combined with 0.5 mg/L BA and 2 mg/L KIN is suitable for shoot multiplication; meanwhile, MS medium supplemented with 2 mg/L NAA improved rooting ability as well as quality of plantlets and to improving survival rate and acclimatization of Gerbera cultured in vitro.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/15672
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2022
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  • 3
    Online Resource
    Online Resource
    Production of in vitro strawberry (Fragaria × ananassa) plantlets in large-scale system supplemented with silver nanoparticles
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2021
    In:  Vietnam Journal of Biotechnology Vol. 19, No. 3 ( 2021-10-13), p. 481-493
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 19, No. 3 ( 2021-10-13), p. 481-493
    Abstract: The growth of strawberry plantlets in the rooting stage on culture medium supplemented with silver nanoparticles (AgNPs) and the ethylene gas accumulation in plantlet culture bottles were investigated. In addition, different culture systems were first used to produce large-scale Strawberry plantlets. The results showed that shoots (3 cm) were cultured on MS medium supplemented with 0.02 mg/L NAA, 1 g/L activated charcoal, 30 g/L sucrose, 8 g/L agar and 0.5 mg/L AgNPs showed about 4 days earlier rooting formation and the plantlet growth such as plantlet height (5.60 cm), fresh weight (242.67 mg), dry weight (34,67 mg), number of roots/plantlet (6.67), root length (3.40 cm), SPAD (39.30 nmol/cm2) were higher than those in the control after 15 days of culture. Besides, the ethylene gas content in the culture bottle (0.06 ppm) in the 0.5 mg/L AgNPs treatment was lower than as compared to that in the control (0.15 ppm) after 15 days of culture. A shoot density (10 shoots) in 250 mL culture bottle with 40 mL of medium gave optimal growth than those in other treatments after 15 days of culture. Square plastic box culture system (length × width × height: 19 cm × 19 cm × 7 cm; 2.5 L in volume) containing 250 mL MS medium added to 0.5 mg/L AgNPs produced 100 vigorous plantlets; meanwhile, rectangular plastic box system (34 cm × 23 cm × 13 cm; 10 L in volume; 10 L in volume) produced 200 vigorous plantlets. Plantlets derived from 0.5 mg/L AgNPs treatment in the plastic box systems exhibited well acclimatization after 30 and 60 days of culture in the greenhouse.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/15491
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2021
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  • 4
    Online Resource
    Online Resource
    High-frequency in vitro shoot regeneration of Saintpaulia ionantha Wendl. by light-emitting diodes
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2022
    In:  Vietnam Journal of Biotechnology Vol. 19, No. 4 ( 2022-05-03), p. 717-724
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 19, No. 4 ( 2022-05-03), p. 717-724
    Abstract: Saintpaulia ionantha Wendl. is a small flower, with a variety of colors, which are commonly grown indoors for decorative purposes. Currently, micropropagation of S. ionantha has been achieved certain results; however, the shoot quality as well as number of shoots regeneration was not high. In this study, the effect of LEDs system on the shoot multiplication and shoot quality of S. ionantha was conducted. The leaves were cut into square patterns without the main veins (1 cm × 1 cm), and the petiole (3 mm in diameter) was cut with a thick layer (2 mm) of longitudinal cell (lTCL), which were used as the initial materials. The explants were placed under LEDs conditions; and white fluorescent tube lamp (FL) was used as the control for shoot regeneration and rooting. The results found that the quality of light affects the regeneration, growth and development of S. ionantha shoots. The LED condition with a combination of 7R + 3B are optimal for high-frequency of shoot regeneration with the regeneration rate: 67.25%, and number of shoots: 9.33 shoots/explant from the leaves without the main veins. The higher shoot multiplication (number of shoots: 10.00 shoots/explant, fresh weight: 150.07 mg, dry weight: 98.64 mg) and rooting rate of plantlet (fresh weight: 2.17 g; dry weight: 0.09 g; leaf number: 20.33 leaves/plantlet) were also obtained under 7R + 3B condition.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/13776
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2022
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  • 5
    Online Resource
    Online Resource
    Effect of factors on growth and development of Dendrobium officinale Kimura et Migo cultured in vitro and ex vitro
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2021
    In:  Vietnam Journal of Biotechnology Vol. 19, No. 2 ( 2021-08-02), p. 321-335
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 19, No. 2 ( 2021-08-02), p. 321-335
    Abstract: Dendrobium officinate Kimura et Migo, a species of orchid for beautiful flowers, is used in traditional medicine in many Asian countries because there are many important pharmaceuticals (chrysotoxene, erianin, confusarin, polysaccharide, alkaloid ...) in anti-cancer, anti-aging, boosting immunity and vasodilation, etc. In recent years, more than 4,000 hectares of Dendrobium officinate Kimura et Migo artificial planting has been available in China and the price of dry product was around ¥ 80,000/kg. Currently, plantlet quality is an important factor influencing the acclimatization stage, growth, and development of plants in the greenhouse. In this study, the effect of a number of factors medium, plant regulator, ventilation, and substrate to improve plantlet quality as well as further growth and development in the greenhouse conditions were investigated. The results showed that SH medium was suitable for in vitro shoot growth in terms of monitoring parameters after 90 days of culture. In shoot multiplication stage, the shoots culture on SH medium supplemented with 2 mg/L BA, 30 g/L sucrose, 9 g/L agar, 1g /L activated charcoal (AC) gave the best results with 4.53 shoots/per shoot. The SH medium supplemented with 1.0 mg/L NAA, 30 g/L sucrose, 9 g/L agar, 1.0 g/L AC combined with ventilation conditions was suitable for rooting stage with plant height (5.73 cm), number of roots (4.77), root length (5.00 cm), fresh weight (3.36 g), dry weight (0,31 g), and total chlorophyll (SPAD) (45.76 nmol/cm2). Plantlets derived from culture ventilation conditions cultivated on the mixture of pine bark and fern fiber (50:50) was the highest survival rate (100%) and growth after 12 months in the greenhouse.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/14543
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2021
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  • 6
    Online Resource
    Online Resource
    Silver nanoparticles enhanced efficiency of explant surface disinfection and somatic embryogenesis in Begonia tuberous via thin cell layer culture
    Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications) ; 2021
    In:  Vietnam Journal of Biotechnology Vol. 19, No. 2 ( 2021-08-02), p. 337-347
    Details
    In: Vietnam Journal of Biotechnology, Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications), Vol. 19, No. 2 ( 2021-08-02), p. 337-347
    Abstract: In vitro culture establishment is one of the most important stages in micropropagation. The disinfectant effectiveness depends on the type of surface disinfectant, concentration and the time treatment. In this initial study, silver nanoparticles (AgNPs) were used as a disinfectant for petioles, flower stalks and stems of Begonia tuberous. In addition, thin cell layer culture (TCL) technique has been applied for the purpose of somatic embryogenesis. The results showed that AgNPs were effective in eliminating infectious microorganisms on B. tuberous explants; which were identified included 4 species of fungi (Fusarium sp., Aspergillus aculeatus, Trichoderma sp. and Penicillium sp.) and 1 species of bacteria (Pseudomonas sp.). At concentrations of 200 ppm and 300 ppm, AgNPs were not only effective in disinfection but also increased the induction rate of somatic embryogenesis in flower stalk TCL explants (approximately 40.00%); a similar effect was observed in stem TCL explants at the same concentration. Meanwhile, for petiole TCL explants, the induction rate of somatic embryogenesis was optimal when using AgNPs at a concentration of 100 - 300 ppm to disinfected the explant. In contrast, at high (400 ppm) or low (50 ppm) concentrations of AgNPs did not play a disinfecting role and stimulated somatic embryogenesis. In addition, explants derived from AgNPs sterilization did not show any abnormalities in somatic embryogenesis with shapes such as globular, heart, torpedo, and cotyledon. AgNPs showed double efficacy in sterilization of explants and improved efficiency of somatic embryogenesis from TCL petioles, flower stalks and stems explants; thus increasing the efficiency micropropagation of B. tuberous.
    Type of Medium: Online Resource
    ISSN: 1811-4989 , 1811-4989
    URL: Article
    DOI: 10.15625/1811-4989/15872
    Language: Unknown
    Publisher: Publishing House for Science and Technology, Vietnam Academy of Science and Technology (Publications)
    Publication Date: 2021
    Location Call Number Limitation Availability
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    Online Resource
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