In:
Journal of the American Society of Nephrology, Ovid Technologies (Wolters Kluwer Health), Vol. 11, No. 9 ( 2000-09), p. 1639-1646
Abstract:
The expression of CD14, CD18, and major histocompatibility complex II on unprimed monocytes from healthy donors after incubation with IgG from patients with antineutrophil cytoplasmic autoantibody (ANCA)-positive active Wegener's granulomatosis ( n = 6) and microscopic polyangiitis ( n = 6) in comparison with IgG from healthy controls ( n = 6) was studied. Monocytes were incubated with IgG (100 μg/ml) at 37°C, and expression of antigens was measured by fluorescence-activated cell sorter after 18 h. Cytoplasmic ANCA (C-ANCA) IgG and perinuclear ANCA (P-ANCA) IgG in comparison with control IgG increased the expression of CD14 (49.2% [SD: 37, P 〈 0.001], and 55.8% [SD: 41, P 〈 0.05]) and CD18 (11.4% [SD: 18, P 〈 0.01] and 8% [SD: 26, P 〈 0.05]) but did not change the major histocompatibility complex II expression. Upregulation of CD14 started after 6 h and reached a peak after 10 to 14 h of incubation and was not inhibited by polymyxin B. F(ab) 2 fragments of C- and P-ANCA IgG also increased expression of CD14 and CD18 as compared with control IgG F(ab) 2 , but for CD14 less than with complete IgG. ANCA IgG depleted of antiproteinase 3 and antimyeloperoxidase antibodies by immunoadsorption failed to upregulate CD14. Monoclonal murine antibodies against proteinase 3 and myeloperoxidase yielded a strong upregulation of CD14 when compared with an isotype control or human control IgG. The data show that CD14 and CD18 are upregulated on monocytes by C- and P-ANCA IgG in vitro , as well as by monoclonal antibodies against proteinase 3 and myeloperoxidase and that this effect is not dependent on Fcγ receptor crosslinking. Upregulation of CD14 and CD18 on monocytes by ANCA suggests a pathogenetic role of ANCA monocyte interactions in systemic vasculitis.
Type of Medium:
Online Resource
ISSN:
1046-6673
DOI:
10.1681/ASN.V1191639
Language:
English
Publisher:
Ovid Technologies (Wolters Kluwer Health)
Publication Date:
2000
detail.hit.zdb_id:
2029124-3
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