In:
Drug Testing and Analysis, Wiley, Vol. 11, No. 9 ( 2019-09), p. 1403-1411
Abstract:
The aims of this study were (a) to develop a liquid chromatography−tandem mass spectrometry (LC–MS/MS) method for the identification of 27 benzodiazepines and metabolites in dried blood spots (DBSs) and in whole blood; (b) to compare the diagnostic reliability of DBSs with blood analyses; and c) to monitor analytes stability on DBSs within a three‐month period. Aliquots of 85 μL of blood from post‐mortem cases were pipetted on cards for DBS analysis. We also collected a tube of blood and stored it at − 20°C. The cards were allowed to dry at room temperature. For each case, DBSs were analyzed immediately (T0), within the following 3 weeks (T1, T2, T3) and after 3 months (T4). The method was applied to 60 post‐mortem cases. A screening procedure was applied to all 27 molecules, while the method was fully validated for the 9 molecules detected in real samples. Limits of detection (LODs) and limits of quantification (LOQs) were in the range 0.1–50.0 ng/mL and 5.0–100.0 ng/mL, respectively. Nine analytes were detected and quantitated in 14 cases: diazepam ( n = 6, 58–162 ng/mL), desmethyldiazepam ( n = 6, 99‐ 〉 500 ng/mL), 7‐aminoclonazepam ( n = 5, 43‐ 〉 500 ng/mL), alprazolam ( n = 2, 15 and 69 ng/mL), chlordesmethyldiazepam ( n = 1, 55 ng/mL), desalkylflurazepam ( n = 1, 270 ng/mL), flurazepam ( n = 1, 50 ng/mL), medazepam ( n = 1, 155 ng/mL), and midazolam ( n = 1, 227 ng/mL). The concentrations on DBSs were in good agreement with those obtained on conventional blood samples ( 〈 20% variation). Except for midazolam (degraded in 1 week), desalkylflurazepam and medazepam (decreased more than 50% after three months), a good stability for a three‐month period was observed for most of the compounds detected in real post‐mortem samples.
Type of Medium:
Online Resource
ISSN:
1942-7603
,
1942-7611
Language:
English
Publisher:
Wiley
Publication Date:
2019
detail.hit.zdb_id:
2462344-1
SSG:
15,3
SSG:
31
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