In:
Journal of Biomolecular NMR, Springer Science and Business Media LLC, Vol. 75, No. 6-7 ( 2021-07), p. 255-272
Abstract:
Progress in NMR in general and in biomolecular applications in particular is driven by increasing magnetic-field strengths leading to improved resolution and sensitivity of the NMR spectra. Recently, persistent superconducting magnets at a magnetic field strength (magnetic induction) of 28.2 T corresponding to 1200 MHz proton resonance frequency became commercially available. We present here a collection of high-field NMR spectra of a variety of proteins, including molecular machines, membrane proteins, viral capsids, fibrils and large molecular assemblies. We show this large panel in order to provide an overview over a range of representative systems under study, rather than a single best performing model system. We discuss both carbon-13 and proton-detected experiments, and show that in 13 C spectra substantially higher numbers of peaks can be resolved compared to 850 MHz while for 1 H spectra the most impressive increase in resolution is observed for aliphatic side-chain resonances.
Type of Medium:
Online Resource
ISSN:
0925-2738
,
1573-5001
DOI:
10.1007/s10858-021-00373-x
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2021
detail.hit.zdb_id:
2006645-4
SSG:
12
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