In:
International Archives of Allergy and Immunology, S. Karger AG, Vol. 96, No. 4 ( 1991), p. 289-295
Abstract:
Multiple mRNA species encoding several predicted forms of the high-affinity IgE receptor α sub-unit (FcεRI-α) have been previously characterized from rat basophilic leukemia cells. Using the polymerase chain reaction procedure, we have extended these findings to show that one FcεRI-α mRNA variant, characterized by a 163-bp deletion within the coding sequence, exists in normal rat connective tissue mast cells as well as in both transformed and non transformed murine mast cell lines. In addition, a partial murine FcεRI-α genomic clone, spanning the internal-deletion sequence, has been identified, and from analysis of this sequence a mechanism of alternative pre-mRNA splicing is proposed. Finally, mRNA variants have been translated in a cell-free system and the protein products partially characterized.
Type of Medium:
Online Resource
ISSN:
1018-2438
,
1423-0097
Language:
English
Publisher:
S. Karger AG
Publication Date:
1991
detail.hit.zdb_id:
1482722-0
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