In:
PLOS ONE, Public Library of Science (PLoS), Vol. 18, No. 3 ( 2023-3-30), p. e0283537-
Abstract:
Zoonotic spillover of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to humans in December 2019 caused the coronavirus disease 2019 (COVID-19) pandemic. Serological monitoring is critical for detailed understanding of individual immune responses to infection and protection to guide clinical therapeutic and vaccine strategies. We developed a high throughput multiplexed SARS-CoV-2 antigen microarray incorporating spike (S) and nucleocapsid protein (NP) and fragments expressed in various hosts which allowed simultaneous assessment of serum IgG, IgA, and IgM responses. Antigen glycosylation influenced antibody binding, with S glycosylation generally increasing and NP glycosylation decreasing binding. Purified antibody isotypes demonstrated a binding pattern and intensity different from the same isotype in whole serum, probably due to competition from the other isotypes present. Using purified antibody isotypes from naïve Irish COVID-19 patients, we correlated antibody isotype binding to different panels of antigens with disease severity, with binding to the S region S1 expressed in insect cells (S1 Sf21) significant for IgG, IgA, and IgM. Assessing longitudinal response for constant concentrations of purified antibody isotypes for a patient subset demonstrated that the relative proportion of antigen-specific IgGs decreased over time for severe disease, but the relative proportion of antigen-specific IgA binding remained at the same magnitude at 5 and 9 months post-first symptom onset. Further, the relative proportion of IgM binding decreased for S antigens but remained the same for NP antigens. This may support antigen-specific serum IgA and IgM playing a role in maintaining longer-term protection, important for developing and assessing vaccine strategies. Overall, these data demonstrate the multiplexed platform as a sensitive and useful platform for expanded humoral immunity studies, allowing detailed elucidation of antibody isotypes response against multiple antigens. This approach will be useful for monoclonal antibody therapeutic studies and screening of donor polyclonal antibodies for patient infusions.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0283537
DOI:
10.1371/journal.pone.0283537.g001
DOI:
10.1371/journal.pone.0283537.g002
DOI:
10.1371/journal.pone.0283537.g003
DOI:
10.1371/journal.pone.0283537.g004
DOI:
10.1371/journal.pone.0283537.g005
DOI:
10.1371/journal.pone.0283537.g006
DOI:
10.1371/journal.pone.0283537.g007
DOI:
10.1371/journal.pone.0283537.g008
DOI:
10.1371/journal.pone.0283537.g009
DOI:
10.1371/journal.pone.0283537.t001
DOI:
10.1371/journal.pone.0283537.t002
DOI:
10.1371/journal.pone.0283537.s001
DOI:
10.1371/journal.pone.0283537.s002
DOI:
10.1371/journal.pone.0283537.s003
DOI:
10.1371/journal.pone.0283537.s004
DOI:
10.1371/journal.pone.0283537.s005
DOI:
10.1371/journal.pone.0283537.s006
DOI:
10.1371/journal.pone.0283537.s007
DOI:
10.1371/journal.pone.0283537.s008
DOI:
10.1371/journal.pone.0283537.s009
DOI:
10.1371/journal.pone.0283537.s010
DOI:
10.1371/journal.pone.0283537.s011
DOI:
10.1371/journal.pone.0283537.s012
DOI:
10.1371/journal.pone.0283537.s013
DOI:
10.1371/journal.pone.0283537.s014
DOI:
10.1371/journal.pone.0283537.s015
DOI:
10.1371/journal.pone.0283537.s016
DOI:
10.1371/journal.pone.0283537.s017
DOI:
10.1371/journal.pone.0283537.s018
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2267670-3
Permalink