In:
Frontiers in Molecular Biosciences, Frontiers Media SA, Vol. 10 ( 2023-3-1)
Abstract:
Genetically-encoded combinatorial peptide libraries are convenient tools to identify peptides to be used as therapeutics, antimicrobials and functional synthetic biology modules. Here, we report the identification and characterization of a cyclic peptide, G4CP2, that interferes with the GAL4 protein, a transcription factor responsible for the activation of galactose catabolism in yeast and widely exploited in molecular biology. G4CP2 was identified by screening CYCLIC, a Yeast Two-Hybrid-based combinatorial library of cyclic peptides developed in our laboratory. G4CP2 interferes with GAL4-mediated activation of galactose metabolic enzymes both when expressed intracellularly, as a recombinant peptide, and when provided exogenously, as a chemically-synthesized cyclic peptide. Our results support the application of G4CP2 in microbial biotechnology and, additionally, demonstrate that CYCLIC can be used as a tool for the rapid identification of peptides, virtually without any limitations with respect to the target protein. The possible biotechnological applications of cyclic peptides are also discussed.
Type of Medium:
Online Resource
ISSN:
2296-889X
DOI:
10.3389/fmolb.2023.1017757
DOI:
10.3389/fmolb.2023.1017757.s001
DOI:
10.3389/fmolb.2023.1017757.s002
DOI:
10.3389/fmolb.2023.1017757.s003
DOI:
10.3389/fmolb.2023.1017757.s004
DOI:
10.3389/fmolb.2023.1017757.s005
DOI:
10.3389/fmolb.2023.1017757.s006
DOI:
10.3389/fmolb.2023.1017757.s007
DOI:
10.3389/fmolb.2023.1017757.s008
DOI:
10.3389/fmolb.2023.1017757.s009
DOI:
10.3389/fmolb.2023.1017757.s010
DOI:
10.3389/fmolb.2023.1017757.s011
DOI:
10.3389/fmolb.2023.1017757.s012
DOI:
10.3389/fmolb.2023.1017757.s013
DOI:
10.3389/fmolb.2023.1017757.s014
DOI:
10.3389/fmolb.2023.1017757.s015
DOI:
10.3389/fmolb.2023.1017757.s016
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2023
detail.hit.zdb_id:
2814330-9
Permalink