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  • 1
    Online Resource
    Online Resource
    New alleles in the mating type determination region of West Atlantic strains of Pseudo-nitzschia multistriata
    Elsevier BV ; 2021
    In:  Harmful Algae Vol. 103 ( 2021-03), p. 101995-
    Details
    In: Harmful Algae, Elsevier BV, Vol. 103 ( 2021-03), p. 101995-
    Type of Medium: Online Resource
    ISSN: 1568-9883
    URL: Article
    DOI: 10.1016/j.hal.2021.101995
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 2099362-6
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Mate Perception and Gene Networks Regulating the Early Phase of Sex in Pseudo-nitzschia multistriata
    MDPI AG ; 2022
    In:  Journal of Marine Science and Engineering Vol. 10, No. 12 ( 2022-12-07), p. 1941-
    Details
    In: Journal of Marine Science and Engineering, MDPI AG, Vol. 10, No. 12 ( 2022-12-07), p. 1941-
    Abstract: Diatoms are photosynthetic microorganisms playing a key role in the functioning of aquatic ecosystems; they are at the base of the food web and are the main drivers of biogeochemical processes. These microalgae have a unique diplontic life cycle in which the vegetative phase entails a cell size reduction that would lead to the extinction of the cell population if the size was not restored, usually by sexual reproduction. The switch from asexual to sexual reproduction needs to be finely synchronized and regulated to ensure its success; to this aim, cells evolved complex chemical crosstalk that mediates mating. We focused our attention on the marine diatom Pseudo-nitzschia multistriata, investigating the reciprocal perception of the opposite mating type (MT) and the genes and signaling molecules putatively involved in the process. From previously available transcriptomic data, we selected a panel of genes deregulated during the early phase of sexual reproduction, confirming for some of them a role during mate perception and establishing a hierarchy governing their behavior. Moreover, we explored the nature of the molecules controlling sexual reproduction in this species, unveiling that the signaling is mediated by the secretion of protein and non-protein cues from the MT− and MT+, respectively. Our results point to a model in which the two MTs stimulate each other, but the stimulation by the MT− is amplified after MT+ perception in a positive feedback manner.
    Type of Medium: Online Resource
    ISSN: 2077-1312
    URL: Article
    DOI: 10.3390/jmse10121941
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2738390-8
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  • 3
    Online Resource
    Online Resource
    An optimised method for intact nuclei isolation from diatoms
    Springer Science and Business Media LLC ; 2021
    In:  Scientific Reports Vol. 11, No. 1 ( 2021-01-18)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 11, No. 1 ( 2021-01-18)
    Abstract: Due to their abundance in the oceans, their extraordinary biodiversity and the increasing use for biotech applications, the study of diatom biology is receiving more and more attention in the recent years. One of the limitations in developing molecular tools for diatoms lies in the peculiar nature of their cell wall, that is made of silica and organic molecules and that hinders the application of standard methods for cell lysis required, for example, to extract organelles. In this study we present a protocol for intact nuclei isolation from diatoms that was successfully applied to three different species: two pennates, Pseudo-nitzschia multistriata and Phaeodactylum tricornutum, and one centric diatom species, Chaetoceros diadema . Intact nuclei were extracted by treatment with acidified NH 4 F solution combined to low intensity sonication pulses and separated from cell debris via FAC-sorting upon incubation with SYBR Green. Microscopy observations confirmed the integrity of isolated nuclei and high sensitivity DNA electrophoresis showed that genomic DNA extracted from isolated nuclei has low degree of fragmentation. This protocol has proved to be a flexible and versatile method to obtain intact nuclei preparations from different diatom species and it has the potential to speed up applications such as epigenetic explorations as well as single cell (“single nuclei”) genomics, transcriptomics and proteomics in different diatom species.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/s41598-021-81238-z
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2615211-3
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  • 4
    Online Resource
    Online Resource
    Engineering the Unicellular Alga Phaeodactylum tricornutum for Enhancing Carotenoid Production
    MDPI AG ; 2020
    In:  Antioxidants Vol. 9, No. 8 ( 2020-08-16), p. 757-
    Details
    In: Antioxidants, MDPI AG, Vol. 9, No. 8 ( 2020-08-16), p. 757-
    Abstract: Microalgae represent a promising resource for the production of beneficial natural compounds due to their richness in secondary metabolites and easy cultivation. Carotenoids feature among distinctive compounds of many microalgae, including diatoms, which owe their golden color to the xanthophyll fucoxanthin. Carotenoids have antioxidant, anti-obesity and anti-inflammatory properties, and there is a considerable market demand for these compounds. Here, with the aim to increase the carotenoid content in the model diatom Phaeodactylum tricornutum, we exploited genetic transformation to overexpress genes involved in the carotenoid biosynthetic pathway. We produced transgenic lines over-expressing simultaneously one, two or three carotenoid biosynthetic genes, and evaluated changes in pigment content with high-performance liquid chromatography. Two triple transformants over-expressing the genes Violaxanthin de-epoxidase (Vde), Vde-related (Vdr) and Zeaxanthin epoxidase 3 (Zep3) showed an accumulation of carotenoids, with an increase in the fucoxanthin content up to four fold. Vde, Vdr and Zep3 mRNA and protein levels in the triple transformants were coherently increased. The exact role of these enzymes in the diatom carotenoid biosynthetic pathway is not completely elucidated nevertheless our strategy successfully modulated the carotenoid metabolism leading to an accumulation of valuable compounds, leading the way toward improved utilization of microalgae in the field of antioxidants.
    Type of Medium: Online Resource
    ISSN: 2076-3921
    URL: Article
    DOI: 10.3390/antiox9080757
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2704216-9
    SSG: 15,3
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